...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Toxicology >Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography-tandem mass spectrometry
【24h】

Targeted lipidomics profiling of acute arsenic exposure in mice serum by on-line solid-phase extraction stable-isotope dilution liquid chromatography-tandem mass spectrometry

机译：通过在线固相萃取稳定同位素稀释液相色谱 - 串联质谱法在小鼠血清中急性砷暴露的初始脂质物质分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipidomics is the area of study dedicated to the comprehensive analysis and characterization of the functions and metabolism of lipids in biological samples. One of the most comprehensively studied classes of lipids is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized from PUFAs and generated majorly from the precursor arachidonic acid. This study identified the profiles of target eicosanoids after acute exposure to arsenic. The principle objective was to determine and validate 10 eicosanoids in mouse serum using on-line solid-phase extraction integrated with liquid chromatography-electrospray tandem mass spectrometry. Intra-day and inter-day repeatability was 82.4-119.2 and 86.7-124.4%, respectively. The limit of detection and limit of quantification were from 0.003 to 0.288 ng/mL and from 0.009 to 0.962 ng/mL, respectively. The levels of 7 of the 10 eicosanoids-namely 8-isoPGF(2 alpha), PGF(2 alpha), PGE(2), 13(s)-HODE, 15(s)-HETE, 12(s)-HETE, and 5(s)-HETE-in mouse serum significantly and dose-dependently increased after arsenic exposure compared with the levels in the vehicle control group. To our knowledge, this is the first study to quantify eicosanoids in mouse serum. This approach provides simple sample preparation, small sample volumes, and a precise and accurate method for determining changes in the profile of these eicosanoids in mouse serum after acute exposure to arsenic.
机译：脂质学是致力于综合分析和表征生物样品中脂质的综合分析和表征的研究领域。最综合学习的脂质类别之一是多不饱和脂肪酸（PUFA）。异氰烷是一系列生物活性脂质介质，其从Pufas代谢并主要由前体arachidonic酸产生。该研究确定了急性暴露于砷后靶籽糖苷的谱。原则目的是使用与液相色谱 - 电喷雾串联质谱法集成的在线固相萃取在小鼠血清中确定和验证10个籽糖苷。日内和日间可重复性分别为82.4-119.2和86.7-124.4％。检测极限和定量限制分别为0.003至0.288ng / ml，分别为0.009至0.962ng / ml。 10个eICosanoids的水平 - 即8-ISOPGF（2α），PGF（2α），PGE（2），13（S）-Hode，15（S） - HETE，12（S），与载体对照组中的水平相比，5（S） - 在砷暴露后显着且剂量依赖性依赖性增加。据我们所知，这是第一次测量小鼠血清中籽糖苷的研究。该方法提供了简单的样品制备，小样品体积和精确和准确的方法，用于在急性暴露于砷后测定小鼠血清中这些七分异素状的轮廓的变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号