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首页> 外文期刊>Indian Journal of Poultry Science >Calpain and calpastatin enzymes in turkey blood and their distribution in breastand thigh muscles
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Calpain and calpastatin enzymes in turkey blood and their distribution in breastand thigh muscles

机译：Calpain和Calpastatin酶在土耳其血液及其在乳腺大腿肌肉中的分布

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摘要

The aim of this study was to compare the concentration of calpain and calpastatin enzymes in blood and their distribution in skeletal muscles of turkey. These enzymes were extracted both from blood and skeletal muscle samples using tris-buffer of 50 mM (pH 6.7), dialyzed at 12 kDa MWCO cellulose filter, and finally, purified and separated on DEAE-Sephacel anion exchange column. Activity analysis was performed using spectrophotometric technique. Results revealed that breast muscle contained significantly higher (P<0.05) /i-calpain concentration as compared to blood and thigh muscles. But m-calpain concentration, was higher in thigh muscle than breast or blood. Concentrations of both the domains of calpain (p.- & -m)were also differed significantly (P<0.05) in blood, breast and thigh muscle samples in between 42 wks and 32 wks age groups of male and female turkey. Significant differences (P<0.05) were also found in calpastatin levels amongst the samples. So, the study envisaged that breast muscle contained highest amount ^-calpain but intermediate concentration of m-calpain and calpastatin. This study is also confirmed the importance of determining both the calpains as well as calpastatin in blood since values generated from this sample has delivering direct information for possible concentration of these enzymes may found in breast and thigh muscles.
机译：本研究的目的是比较血液中鲤鱼和钙钾酶的浓度及其在土耳其骨骼肌中的分布。使用50mm（pH6.7）的TRIS缓冲液，在12kDa MWCO纤维素过滤器透析，最后，在DEAE-Sephacel阴离子交换柱上透析，从血液和骨骼肌样品中提取这些酶。使用分光光度法进行活性分析。结果显示，与血液和大腿肌肉相比，乳房肌肉含有显着更高（P <0.05）/ i-Calpain浓度。但是m-calpain浓度，大腿肌肉高于乳房或血液。钙PAIN（P.-＆-M）的浓度也有显着（P <0.05）在42周之间的血液，乳腺癌和大腿肌肉样品中均有不同（P <0.05）。在样品中，也发现了钙钾素水平的显着差异（P <0.05）。因此，该研究设想，乳房肌肉含有最高量^ -calpain，但中间浓度为m-calpain和calpastatin。本研究还证实了确定血液中Calpains以及胰酸类的重要性，因为从该样品产生的值提供了在乳腺和大腿肌肉中发现这些酶可能浓度的直接信息。

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  • 来源
    《Indian Journal of Poultry Science》 |2016年第2期|共6页
  • 作者

    KRIPRIYALINI L; ASHIM KUMAR BISWAS; SAMARTH TANDON; C.K. BEURA; A.S.YADAV;

  • 作者单位

    Division of Post-Harvest Technology ICAR-Central Avian Research Institute Izatnagar Bareilly-243122(UP);

    Division of Post-Harvest Technology ICAR-Central Avian Research Institute Izatnagar Bareilly-243122(UP);

    Division of Post-Harvest Technology ICAR-Central Avian Research Institute Izatnagar Bareilly-243122(UP);

    Division of Post-Harvest Technology ICAR-Central Avian Research Institute Izatnagar Bareilly-243122(UP);

    Division of Post-Harvest Technology ICAR-Central Avian Research Institute Izatnagar Bareilly-243122(UP);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 家禽;
  • 关键词

    Calpain; calpastatin; turkey meat; tenderization; blood;

    机译：Calpain;Calpastatin;土耳其肉;嫩化;血液;

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