...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Food Microbiology >Characterization of LuxI/LuxR and their regulation involved in biofilm formation and stress resistance in fish spoilers Pseudomonas fluorescens
【24h】

Characterization of LuxI/LuxR and their regulation involved in biofilm formation and stress resistance in fish spoilers Pseudomonas fluorescens

机译：鲁西/鲁尔的特征及其参与生物膜形成和鱼类扰流板的胁迫性荧光荧光荧光荧光素

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Quorum sensing (QS) is crucial for adaption and development of foodborne bacteria in diverse environments. Pseudomonas fluorescens PF07 with QS mediated acylated homoserine lactones (AHLs) activity was isolated from spoiled large yellow croaker (Pseudosciaena crocea). In this study AHL-mediated QS system was characterized and their roles in biofilm formation, motility, stress response and spoilage of P. fluorescens were evaluated. A LuxI/LuxR homolog consisting of a conserved AHL synthase gene (luxI) and a transcriptional regulator gene (luxR) was identified in the strain. Two in-frame deletion mutants of luxI and luxR, Delta luxI and Delta luxR, were constructed to explore their QS signaling function in P. fluorescens. Three types of AHLs were detected in PF07 culture by LC-MS/MS, and N-butanoyl-L-homoserine lactone (C-4-HSL) was a major signal molecule. The C-4-HSL activities was almost abolished in Altai, and decreased greatly in Delta luxR. Compared with wild type (WT) strain, both Delta luxI and Delta luxR showed the significant decrease of biofilm biomass and expolysaccharide production, resulting in thinner and incompact biofilm structure, but promoted swimming motility. The resistance of P. fluorescens to H2O2, heat, NaCl and crystal violet apparently declined in two mutants compared to WT. Spoilage factors, siderophore and protease, apparently attenuated due to deletion of luxI or luxR gene, while the growth and TVB-N production did not differ. Furthermore, the changes of the biofilm formation, motility and protease in Delta luxI strain were partially restored by the exogenous C-4-HSL. In agreement with the effect of two mutants on various phenotypes, the transcriptions of alg, lapA, figA, rpoS, and aprX were significantly down-regulated, and figA was up-regulated in Delta luxI and Delta luxR. Therefore, the present study highlighted that the co-operation of LuxI/LuxR homolog was an important QS regulator in biofilm formation, motility and spoilage potential, and hinted its positive regulation of stress resistance with RpoS in P. fluorescens.
机译：法定感测（QS）对于各种环境中食源性细菌的适应和开发至关重要。用QS介导的酰化酰化的杂环内酯（AHLS）活性的假单胞菌荧光荧光素PF07从被腐败的大黄色克罗斯克（假血症鳄鱼）中分离出来。在本研究中，评估了AHL介导的QS系统，并评估了P.荧光的生物膜形成，运动，应力反应和腐败的作用。在菌株中鉴定出由保守的AHL合成酶基因（LUXI）和转录调节剂基因（LUXR）组成的LUXI / LUXR同源物。建造了两个框架和Luxr，Delta Luxi和Delta Luxr的框架内缺失突变体，以探索P.荧光的QS信号功能。通过LC-MS / MS在PF07培养中检测到三种AHL，并且N-丁酰基-L-HOMOSERINE内酯（C-4-HSL）是主要信号分子。 C-4-HSL活动几乎废除在阿尔泰，并在三角洲Luxr下降了大大减少。与野生型（WT）菌株相比，Delta Luxi和Delta Luxr都显示出生物膜生物质和渗透性产生的显着降低，导致较薄和致力于生物膜结构，但促进了游泳运动。与WT相比，在两个突变体中，P.荧光荧光至H 2 O 2的抗性显然下降。腐败的因素，纵横道和蛋白酶，显然因缺失鲁西或鲁尔基因而衰减，而增长和TVB-N的生产没有差异。此外，通过外源C-4-HSL部分恢复Delta Luxi菌株中生物膜形成，运动和蛋白酶的变化。同意，对于两个突变体对各种表型的影响，ALG，LAPA，FIGA，RPO和APRX的转录显着下调，图形在DELTA LUXI和DELTA LUXR中调节。因此，本研究强调，Luxi / Luxr Homolog的合作是生物膜形成，运动和腐败潜力的重要QS调节剂，并暗示其在P.荧光中与RPO的应力抗性调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号