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首页> 外文期刊>International Journal of Horticulture & Crop Science Research >Somatic Embryogenesis and Plant Regeneration from Hypocotyl Explant of Green Gram (Vigna Radiata L) - A Recalcitrant Grain Legume
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Somatic Embryogenesis and Plant Regeneration from Hypocotyl Explant of Green Gram (Vigna Radiata L) - A Recalcitrant Grain Legume

机译：绿革氏胚乳植物的体细胞胚胎发生和植物再生（Vigna Radiata L） - 一种顽固的谷物豆类

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摘要

A protocol for plant regeneration via somatic embryogenesis was developed in two high yielding green gram cultivar TAP-7 & Pusabaisaki. Somatic embryos were induced from hypocotyl explants on MS medium fortified with different concentrations of 2, 4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), a- naphthalene acetic acid (NAA) and picloram either singly or in combination with 0.5-1.5 mg/1 N6-benzylaminopurine (BAP) or 0.5-1.5 mg/1 kinetin (KN). Type of auxin, its concentration and genotype influenced the frequency of somatic embryogenesis. NAA was better for somatic embryo induction compared to other auxins. The well formed, cotyledonary shaped embryos germinated into plantlets with 62.4% frequency on MS medium supplemented with 0.5 mg/1 BAP + 0.75 mg/1 ABA. The frequency of embryogenesis and subsequent plantlet regeneration was higher in TAP-7 than in Pusabaisaki. Regenerated plants were transferred to soil and grown to maturity with 30% survival. This protocol to produce embryos with a high frequency and their subsequent conversion to whole plants offers potential for gene transfer and development of transgenics in this important grain legume.
机译：通过体细胞胚胎发生的植物再生协议，在两个高产绿革革处理TAP-7和PUSABAISAKI中开发。从培解的MS培养基中诱导体细胞胚，强化不同浓度的2,4-二氯苯乙烯酸（2,4-D），2,4,5-三氯苯乙烯酸（2,4,5-T），A-萘乙酸（NAA）和糠烷为单独或与0.5-1.5mg / 1 n6-苄氨基嘌呤（BAP）或0.5-1.5mg / 1 kinetin（kN）组合。养肝型，其浓度和基因型影响了体细胞胚胎发生的频率。与其他助体素相比，NAA对于体细胞胚胎诱导更好。形成良好的子叶形状的胚胎萌发到植物中，在30.4％的频率上，在补充有0.5mg / 1bap + 0.75mg / 1ab的MS培养基上。胚胎发生的频率和随后的小植物再生在TIP-7中高于Pusabaisaki。再生植物转移到土壤中并以30％存活的成熟度成熟。该方案以高频和其随后转化为整株植物的胚胎提供了该重要谷物豆类中基因转移和转基因的潜力。

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