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首页> 外文期刊>Electroanalysis >Label-Free and Ultra-Low Level Detection of Salmonella enterica Serovar Typhimurium Using Electrochemical Impedance Spectroscopy
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Label-Free and Ultra-Low Level Detection of Salmonella enterica Serovar Typhimurium Using Electrochemical Impedance Spectroscopy

机译:使用电化学阻抗光谱法无标记和超低水平检测沙门氏菌肠道血轮训练器

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摘要

An immunosensor for rapid and low level detection of the bacterial pathogen Salmonella enterica Serovar Typhimurium was designed and developed based upon label-free electrochemical impedance spectroscopy and correlated to viable cell counts. The immunosensor was fabricated by electroplating gold onto a disposable printed circuit board (PCB) electrode by immobilizing monoclonal antibody (MAb) specific against Salmonella typhimurium cell surface lipopolysaccharide (LPS) onto the surface of the electrode. Use of mass-fabricated and electroplated PCB electrodes allowed for disposable, highly sensitive, and rapid detection of Salmonella in an aqueous environment. Results demonstrate that in purified solution, Salmonella can be detected as low as 10 CFU in a 100 mu L volume and label-free and rapid manner in fewer than 90 s. The cost effective approach described here can be used for detection of pathogens with relevance for healthcare, food, and environmental applications.
机译:基于无标记的电化学阻抗光谱,设计和开发了一种免疫传感器,用于基于无标记的电化学阻抗光谱,并与可活细胞计数相关并与活性细胞计数相关的快速和低水平检测。 通过将单克隆抗体(MAB)固定在电极的表面上,通过将单克隆抗体(MAB)固定在电极的表面上,通过将单克隆抗体(MAB)固定到一次性印刷电路板(PCB)电极上,通过将单克隆抗体(MAB)固定到所述电极的表面上来制造免疫传感器。 使用大规模制造和电镀PCB电极允许一次性,高度敏感,快速地检测水分环境中的沙门氏菌。 结果表明,在纯化的溶液中,可以在100μl体积的100μl体积和无标记和快速的方式中检测到10个CFU的低至10℃。 这里描述的成本有效的方法可用于检测具有医疗保健,食品和环境应用相关性的病原体。

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