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首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Reproducible measurement of vancomycin MICs within the susceptible range in Staphylococcus aureus by a broth microdilution method with a “quasi-continuum” gradient of antibiotic concentrations
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Reproducible measurement of vancomycin MICs within the susceptible range in Staphylococcus aureus by a broth microdilution method with a “quasi-continuum” gradient of antibiotic concentrations

机译：用肉汤微量稀释法用“准连续素”抗生素浓度的肉毒杆菌易感范围内易感范围内的易感测量

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摘要

Abstract The availability of reproducible broth microdilution (BMD) methods including inter log 2 antibiotic dilutions for measuring Staphylococcus aureus (SA) vancomycin minimum inhibitory concentrations (MICs) within the susceptible range is needed to elucidate the impact of vancomycin MICs on clinical outcomes of invasive SA infections. Here, we report on the development of a very precise BMD method that incorporates the following incremental antibiotic concentrations: 0.50, 0.62, 0.75, 0.87, 1.0, 1.25, 1.40, 1.50, 1.60, 1.75, and 2.0?μg/mL. The intra- and inter-assay coefficients of variation of this method were around 20%. The mean of the differences in MIC values for all isolates obtained across two independent runs performed at one center was 0.04?μg/mL [95% confidence interval (CI), 0.011–0.07?μg/mL] and that for ten isolates measured at two different centers was 0.04?μg/mL (95% CI, 0–13?μg/mL). Vancomycin MIC values differed by less than 0.1?μg/mL between runs for most isolates. Storage of isolates at ?20?°C for up to 3?months had no impact on the vancomycin MIC values. The mean vancomycin MIC values obtained by the Etest using a standard inoculum (0.5 McFarland) were significantly higher ( p ?≤ 0.001) than those measured by BMD and the MIC values measured by the two methods correlated poorly (Rho, 0.319; p ?=?0.148). Nevertheless, the mean MIC values measured by the Etest using lower inocula (10 7 or 10 6 ?CFU/mL) and those measured by BMD were comparable and correlated significantly ( p ?=?0.004 for 10 7 ?CFU/mL and p ?=?0.029 for 10 6 ?CFU/mL).
机译：摘要可重复的肉汤微量稀释（BMD）方法，包括用于测量易感范围内的葡萄球菌（SA）万古霉素（SA）万古霉素最低抑制浓度（MIC）的抗生素稀释液的可用性，以阐明Vancomycin MIC对侵袭性SA临床结果的影响感染。在这里，我们报告了一种非常精确的BMD方法的开发，其包含以下增量抗生素浓度：0.50,0.62,0.75,0.87,1.0,1.25,1.40,1.50,1.60,1.75和2.0≤μg/ ml。该方法的变异的分析阶段和分析系数约为20％。在一个中心进行的两个独立运行中获得的所有分离物的差异的平均值为0.04Ω·μg/ ml [95％置信区间（CI），0.011-0.07Ω·μg/ ml]，并且在此处测量的十个分离物两个不同的中心为0.04Ω·μg/ ml（95％CI，0-13×μg/ mL）。万古霉素MIC值在大多数隔离物之间的运行之间不同的差异小于0.1Ωμg/ ml。在Δ20？°C储存最多3个月的储存对万古霉素MIC值没有影响。通过使用标准接种物（0.5mcFarland）的ETEST获得的平均万霉素MIC值显着高于（p≤0.001），而不是通过BMD测量的那些，并且通过两种方法测量的MIC值相关（RHO，0.319; P？= ？0.148）。然而，使用下部接种物（10 7或10 6→CFU / ml）通过ETEST测量的平均麦克风值和通过BMD测量的那些是可比的并且显着相关（p？= 0.004，10 7？CFU / ml和P？ = 0.029持续10 6？CFU / ml）。

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