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首页> 外文期刊>European Journal of Plant Pathology >Enhancement of PR1 and PR5 gene expressions by chitosan treatment in kiwifruit plants inoculated with Pseudomonas syringae pv. actinidiae
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Enhancement of PR1 and PR5 gene expressions by chitosan treatment in kiwifruit plants inoculated with Pseudomonas syringae pv. actinidiae

机译：用Pseudomonas inringae PV接种的壳聚糖植物中壳聚糖治疗的PR1和PR5基因表达的增强。 actinidiae.

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摘要

Kiwifruit, with a production of more than 1.5 million tons/year in the world, must be protected against attack by its most common pathogen. Following the European guidelines on the substitution of pesticides by safer alternatives, the aim of this work was to verify if kiwifruit plants are able to better resist pathogen infections through the use of chitosan, a biodegradable compound and a well-known elicitor of Systemic Acquired Resistance (SAR). To evaluate the chitosan's elicitation effect in plant during the treatment period, two genes involved in the metabolic pathway of SAR were chosen, Pathogenesis Related Protein 1 and 5 (PRs). Primers for both genes were designed and validated and chitosan's elicitation effect was tested in qRT-PCR. Elicitation of SAR was first evaluated in a model system with plants cultured in vitro and subsequently in 2 year old plants belonging to two different species (Actinidia chinensis Planch. and A. deliciosa (A. Chev.) C.F. Liang & A.R. Ferguson). To evaluate the effects of chitosan elicitation in the presence of the pathogen attack, the 2-year-old plants were inoculated with the bacterium Pseudomonas syringae pv. actinidiae. Micropropagated kiwifruit plants were a good model to test molecular markers for SAR onset. Moreover, PR1 and PR5 have also shown to be suitable candidates for the detection of the plant immune system activation. In this study, chitosan elicited a systemic response in kiwi plants with intensity comparable to other well-known signalling compounds (salicylic acid, methyl jasmonate or ethylene), as shown by the changes in PR1's and PR5's transcription profiles. The data obtained by chitosan treatments in in vitro cultures were confirmed in plants grown in greenhouse, in which, moreover, the combination of chitosan treatment and the bacterial inoculum had the greatest effect on PRs synthesis. This study also proved that chitosan, leading to an increased expression of both PRs, has a role in kiwifruit defense reactions.
机译：在世界上生产超过150万吨/年的猕猴桃，必须受到最常见的病原体的影响。在通过更安全的替代品替代农药的欧洲准则之后，这项工作的目的是通过使用壳聚糖，可生物降解的化合物和全身性获得的抗性的公知的引导者来验证Kiwifruit植物是否能够更好地抵抗病原体感染（SAR）。为了评估治疗期间植物中的壳聚糖的诱导效果，选择了SAR代谢途径的两种基因，发病机制相关蛋白1和5（PRS）。设计和验证了两个基因的引物，并在QRT-PCR中测试了壳聚糖的诱导效果。首先在具有体外培养的植物的模型系统中评估SAR的诱导，随后在属于两种不同物种的2岁植物（Actinidia Chinensis Planch。和A. Deliciosa（A.Chev.）C.f.梁＆amp; A.R. Ferguson）。为了评估壳聚糖诱导在病原体攻击存在下的影响，用细菌假鼠霉素PV接种了2岁的植物。猕猴桃。微催化的猕猴桃植物是测试SAR发作的分子标记的良好模型。此外，PR1和PR5还显示出用于检测植物免疫系统活化的合适候选者。在这项研究中，壳聚糖引发了猕猴桃植物的全身反应，其强度与其他众所周知的信号化合物（水杨酸，茉莉酸甲酯或乙烯）相当，如PR1和PR5的转录谱的变化所示。在温室生长的植物中证实了通过壳聚糖治疗的体外培养物中获得的数据，此外，壳聚糖治疗和细菌接种物的组合对PRS合成具有最大的影响。这项研究还证明了壳聚糖，导致对PRS的表达增加，在Kiwifruit防御反应中具有作用。

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