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Anti-Trypanosoma activity of bioactive metabolites from Photorhabdus luminescens and Xenorhabdus nematophila

机译:来自光隆的生物活性代谢物的抗脑肿瘤活性和Xenorhabdus nematophila

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摘要

Only two drugs are currently available for the treatment of Chagas disease and their effectiveness are unsatisfactory. Photorhabdus luminescens and Xenorhabdus nematophila, two enteric bacteria highly pathogenic to a broad range of insects, have been studied as potential source for bioactive metabolites against protozoa causing neglected tropical diseases. Therefore, we tested the in vitro anti-Trypanosoma cruzi activity of secreted metabolites from these bacteria. The conditioned medium of X. nematophila and P. luminescens showed significant parasiticidal activity in a concentration-dependent manner (IC50 XN = 0.34 mg/mL, IC50 PL = 1.0 mg/mL). The parasiticidal compound was identified as a small molecule stable to heating and pH changes ranging from 2 to 12. Moreover, anti-Trypanosoma molecules secreted by both bacteria stimulate the trypanocidal activity of macrophages by a mechanism independent of nitric oxide. Summarizing, our studies reveal that P. luminescens and X. nematophila are potential sources of putative novel drugs against Chagas disease.
机译:目前只有两种药物目前可用于治疗Chagas疾病,其有效性是不满意的。 Photorhabdus Luminescens和Xenorhabdus nematophila,两种肠道细菌高度致病到广泛的昆虫,已被研究作为生物活性代谢产物的潜在来源,以导致忽视的热带疾病。因此,我们测试了来自这些细菌的分泌分泌的代谢物的体外抗锥体瘤Cruzi活性。 X.Nematophila和P. Luminescens的调节培养基以浓度依赖性方式显示出显着的寄生活性(IC50 xn = 0.34mg / ml,IC 50 PL = 1.0mg / ml)。寄生的化合物被鉴定为稳定于加热的小分子,并且pH从2-12的pH变化。此外,两种细菌分泌的抗锥虫眼分子通过独立于一氧化氮的机制刺激巨噬细胞的胰蛋白酶活性。总结,我们的研究表明,P. Luminescens和X.Nematophila是针对抗噬病疾病的推定新药物的潜在来源。

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