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首页> 外文期刊>Genome >Challenges for assessing vertebrate diversity in turbid Saharan water-bodies using environmental DNA
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Challenges for assessing vertebrate diversity in turbid Saharan water-bodies using environmental DNA

机译：利用环境DNA评估混浊撒哈拉水体中脊椎动物多样性的挑战

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摘要

The Sahara desert is the largest warm desert in the world and a poorly explored area. Small water-bodies occur across the desert and are crucial habitats for vertebrate biodiversity. Environmental DNA (eDNA) is a powerful tool for species detection and is being increasingly used to conduct biodiversity assessments. However, there are a number of difficulties with sampling eDNA from such turbid water-bodies and it is often not feasible to rely on electrical tools in remote desert environments. We trialled a manually powered filtering method in Mauritania, using pre-filtration to circumvent problems posed by turbid water in remote arid areas. From nine vertebrate species expected in the water-bodies, four were detected visually, two via metabarcoding, and one via both methods. Difficulties filtering turbid water led to severe constraints, limiting the sampling protocol to only one sampling point per study site, which alone may largely explain why many of the expected vertebrate species were not detected. The amplification of human DNA using general vertebrate primers is also likely to have contributed to the low number of taxa identified. Here we highlight a number of challenges that need to be overcome to successfully conduct metabarcoding eDNA studies for vertebrates in desert environments in Africa.
机译：撒哈拉沙漠是世界上最大的温暖沙漠，探索众多。小型水体发生在沙漠中，是脊椎动物生物多样性的关键栖息地。环境DNA（EDNA）是物种检测的强大工具，越来越多地用于进行生物多样性评估。然而，从这种混浊的水体采样EDNA存在许多困难，并且依赖于偏远的沙漠环境中的电动工具通常是不可行的。我们在毛里塔尼亚试验了手动供电的过滤方法，使用预过滤来规避遥远干旱地区浑浊水引起的避难所问题。从预期在水体中预期的九种脊椎动物物种，通过两种方法，通过两种方法检测到四种。困难过滤浊度水导致严重的约束，限制了每个研究现场的一个采样点的抽样方案，单独可能在很大程度上解释为什么未检测到许多预期的脊椎动物种类。使用一般脊椎动物引物的人DNA的扩增也可能导致鉴定的少数征收株。在这里，我们突出了许多需要克服的挑战，以成功地对非洲的沙漠环境中的脊椎动物进行eDNA研究。

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  • 来源
    《Genome》 |2018年第11期|共8页
  • 作者

    Egeter Bastian; Peixoto Sara; Brito Jose C.; Jarman Simon; Puppo Pamela; Velo-Anton Guillermo;

  • 作者单位

    Univ Porto Inst Ciencias Agr Vairao Ctr Invest Biodiversidade &

    Recursos Genet CIBIO InBIO R Padre Armando Quintas 7 P-4485661 Vairao Portugal;

    Univ Porto Inst Ciencias Agr Vairao Ctr Invest Biodiversidade &

    Recursos Genet CIBIO InBIO R Padre Armando Quintas 7 P-4485661 Vairao Portugal;

    Univ Porto Inst Ciencias Agr Vairao Ctr Invest Biodiversidade &

    Recursos Genet CIBIO InBIO R Padre Armando Quintas 7 P-4485661 Vairao Portugal;

    Curtin Univ Trace &

    Environm DNA TrEnD Lab Mol &

    Life Sci Bentley WA 6102 Australia;

    Univ Porto Inst Ciencias Agr Vairao Ctr Invest Biodiversidade &

    Recursos Genet CIBIO InBIO R Padre Armando Quintas 7 P-4485661 Vairao Portugal;

    Univ Porto Inst Ciencias Agr Vairao Ctr Invest Biodiversidade &

    Recursos Genet CIBIO InBIO R Padre Armando Quintas 7 P-4485661 Vairao Portugal;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;医学遗传学;
  • 关键词

    eDNA; spike; Mauritania; vertebrate; turbid; desert; biodiversity;

    机译：埃德纳;穗;毛里塔尼亚;脊椎动物;混浊;沙漠;生物多样性;

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