...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEMS Microbiology Letters >An Arf-GAP promotes endocytosis and hyphal growth of Ashbya gossypii
【24h】

An Arf-GAP promotes endocytosis and hyphal growth of Ashbya gossypii

机译：Arf-Gap促进Ashbya Gossypii的内吞作用和悬垂生长

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is important for hyphal growth, we studied the Ashbya gossypii homolog of Saccharomyces cerevisiae ARF3 along with its putative GEF and GTPase-activating protein (GAP) encoded by YEL1 and GTS1, respectively. Deletion of YEL1 had no discernible phenotype and deletion of ARF3 had only a minor defect in vacuolar fusion. In contrast, deletion of GTS1 severely impaired hyphal growth, and mutants showed defects in the maintenance of polarity and the localization of cortical actin patches. The uptake of the lipophilic dye FM4-64 was delayed in gts1 hyphae, indicating a defect in endocytosis. Gts1 has several protein domains, of which the Arf-GAP domain is required for complementation of the gts1 mutant phenotype. GFP-tagged GTS1 under control of its endogenous promoter localized to the plasma membrane but was enriched at hyphal tips and septal sites corresponding to a role in polarized vesicle trafficking. Our results indicate that this ARF-GTPase module plays an important role for filamentous hyphal growth.
机译：ADP-核糖基化因子（ARF）GTP酶的家族高度保守于酵母和调节囊泡芽孢囊。含有蛋白质的SEC7结构域刺激ARF蛋白的鸟嘌呤核苷酸交换，而ARF-GTP酶活化蛋白质刺激GTP的水解。由于小囊泡贩运对于亚腿生长很重要，因此我们将Saccharomyces Cerevisiae ARF3的Ashbya Gossypii同源物与其推定的GEF和GTP酶活性分别由Yel1和GTS1编码。缺乏Yel1没有可辨别的表型，ARF3的缺失只有次要缺陷在真空融合中。相比之下，GTS1严重受损的菌丝生长受损，突变体在维持极性和皮质肌动蛋白贴片的定位方面表现出缺陷。亲脂性染料FM4-64的摄取延迟在GTS1菌丝中，表明内吞作用的缺陷。 GTS1具有几个蛋白质结构域，其中GTS1突变表型的互补需要ARF-GAP结构域。通过控制其内源性启动子的GFP标记的GTS1，其定位于质膜，但在对应于偏振囊泡贩运中的作用的亚乳蛋白尖端和隔膜位点富集。我们的结果表明，该ARF-GTPASE模块对丝状缘生长起着重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号