...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food & Function >Antioxidant and anti-aging effects of a sea cucumber protein hydrolyzate and bioinformatic characterization of its composing peptides
【24h】

Antioxidant and anti-aging effects of a sea cucumber protein hydrolyzate and bioinformatic characterization of its composing peptides

机译：海参蛋白水解产物的抗氧化剂和抗衰老作用及其组成肽的生物信息

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sea cucumbers have been used as food delicacies and traditional medicine for centuries, and their health benefits are partly attributed to their repertoire of proteins. Peptides prepared from the sea cucumberApostichopus japonicusare reported to havein vitroantioxidant activities. Here, we investigated thein vivoantioxidant capacity of AjPH, a peptide-richA. japonicusprotein hydrolyzate, and found that AjPH is capable of increasing the survival rate and reducing the reactive oxygen species (ROS) level in the animal modelCaenorhabditis elegansunder increased oxidative stress induced by paraquat. AjPH is also shown to enhance the antioxidant defense system in paraquat-exposed nematodes, including upregulation of superoxide dismutase and catalase activities and reduction of malondialdehyde contents. To explore underpinning antioxidant mechanisms, cellular and chemical assays were used to demonstrate that AjPH not only reduces ROS accumulation in cells but also directly scavenges DPPH free radicals. Further studies indicate that AjPH can decrease age pigments and extend lifespan but does not reduce food intake, body length and brood size of the nematodes, demonstrating its capacity to delay physiological aging. Using activity-guided fractionation by ultrafiltration and gel filtration, we then isolated antioxidant fractions from AjPH and identified the sequences of their composing peptides, which were subjected toin silicoanalysis for prospective motifs, physicochemical properties and antioxidant potential. Taken together, our results provide an insight into the nutraceutical potential of the sea cucumber protein hydrolyzate for aging and related conditions and also a basis for future mechanistic studies of individual antioxidant peptides.
机译：海参已被用作食物美食和传统医学的几个世纪以来，他们的健康益处部分归因于他们的蛋白质的曲目。从海参中制备的肽据据据据据据据据报道，患有耐族氧化剂活性。在这里，我们研究了Ajph，肽 - Richa的诱发体内氧化能力。日本蛋白蛋白水解产物，发现AJPH能够提高生存率并降低动物型紫杉醇胶质炎的动物模型中的活性氧物质（ROS）水平增加。 AJPH还显示出在百草枯暴露的线虫中增强抗氧化防御系统，包括超氧化物歧化酶和过氧化酯活性的上调和降低丙二醛含量。为了探索支撑抗氧化机制，使用细胞和化学测定来证明AJPH不仅降低了细胞中的ROS积累，而且还直接清除DPPH自由基。进一步的研究表明，AJPH可以减少年龄颜料并延长寿命，但不会减少线虫的食物摄入，体长和育龄尺寸，证明其延迟生理老化的能力。通过超滤和凝胶过滤使用活动引导的分馏，然后从AJPH中分离抗氧化级分，并鉴定了它们的组成肽的序列，其对预期基序，物理化学性质和抗氧化剂潜力进行了硅分析。我们的结果占据了，对海参蛋白质水解产物的营养潜力进行了深入洞察，用于老化和相关条件，以及个体抗氧化肽的未来机械研究的基础。

著录项

  • 来源
    《Food & Function》 |2020年第6期|共13页
  • 作者

  • 作者单位
  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 食品工业;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号