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Rapid biofilm eradication of the antimicrobial peptide 1018-K6 against Staphylococcus aureus: A new potential tool to fight bacterial biofilms

机译:快速生物膜消除抗微生物肽1018-k6对抗金黄色葡萄球菌:用于对抗细菌生物膜的新潜在工具

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Staphylococcal food poisoning - caused by certain enterotoxigenic staphylococci - is one of the most common worldwide foodborne diseases. In this context, bacterial biofilms on food processing plan surfaces can represent critical sources of contamination, being more resistant to cleaning and disinfection procedures. Therefore, it is important to prevent and control biofilm formation in food facilities. In the last years, antimicrobial peptides (AMPs) have emerged as promising biofilm contrasting tools. The recently developed AMP, named 1018-K6, revealed a significant bactericidal (MBC 10 mu M) and biofilm-preventing efficiency (MBIC 50 mu M) against a reference ATCC strain of Listeria monocytogenes and remarkable stability under different environmental conditions. In this work, a kinetic of action of 1018-K6 against two strong biofilm-producing reference strains of Staphylococcus aureus (including a methicillin-resistant S. aureus strain) and a moderate biofilm producer enterotoxigenic S. aureus isolated from cheese, was performed. The peptide showed an impressive rapid mode of action, eradicating established biofilms within few minutes. Bactericidal activity against planktonic cells and inhibition of biofilm formation were also observed. The significant properties of 1018-K6 make it a promising candidate for applications in food-safety and quality control.
机译:葡萄球菌食物中毒 - 由某些肠毒素葡萄球菌引起的 - 是全球最常见的全球食源性疾病之一。在这种情况下,食品加工面表面上的细菌生物膜可以代表污染的关键来源,更耐清洁和消毒程序。因此,重要的是在食品设施中预防和控制生物膜形成。在过去几年中,抗菌肽肽(AMPs)已成为具有前景的生物膜对比工具。最近开发的放大器,名为1018-K6,揭示了显着的杀菌(MBC10μm)和生物膜防止效率(MBIC50μm),免受李斯特菌单核细胞增生的参考ATCC菌株和不同环境条件下的显着稳定性。在这项工作中,进行了1018-k6的动力,其对两种强生生物膜产生的葡萄球菌(包括甲氧西林耐药菌株)和中等生物膜生产商肠毒素S. aureus的作用。肽显示出令人印象深刻的快速作用模式,在几分钟内消除已建立的生物膜。还观察到对浮游细胞的杀菌活性和生物膜形成的抑制。 1018-K6的显着性质使其成为食品安全和质量控制中的应用的有希望的候选者。

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