...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Methods: A Companion to Methods in Enzymology >Using DNase Hi-C techniques to map global and local three-dimensional genome architecture at high resolution
【24h】

Using DNase Hi-C techniques to map global and local three-dimensional genome architecture at high resolution

机译：使用DNASE HI-C技术在高分辨率下映射全局和局部三维基因组架构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The folding and three-dimensional (3D) organization of chromatin in the nucleus critically impacts genome function. The past decade has witnessed rapid advances in genomic tools for delineating 3D genome architecture. Among them, chromosome conformation capture (3C)-based methods such as Hi-C are the most widely used techniques for mapping chromatin interactions. However, traditional Hi-C protocols rely on restriction enzymes (REs) to fragment chromatin and are therefore limited in resolution. We recently developed DNase Hi-C for mapping 3D genome organization, which uses DNase I for chromatin fragmentation. DNase Hi-C overcomes RE-related limitations associated with traditional Hi-C methods, leading to improved methodological resolution. Furthermore, combining this method with DNA capture technology provides a high-throughput approach (targeted DNase Hi-C) that allows for mapping fine scale chromatin architecture at exceptionally high resolution. Hence, targeted DNase Hi-C will be valuable for delineating the physical landscapes of cis-regulatory networks that control gene expression and for characterizing phenotype-associated chromatin 3D signatures. Here, we provide a detailed description of method design and step-by-step working protocols for these two methods. (C) 2018 Elsevier Inc. All rights reserved.
机译：细胞核中染色质的折叠和三维（3D）组织致力于影响基因组功能。过去十年目睹了划定3D基因组结构的基因组工具的快速进展。其中，基于染色剂的染色捕获（3C），如HI-C是用于测绘染色质相互作用的最广泛使用的技术。然而，传统的HI-C方案依赖于限制酶（RES）至片段染色质，因此在分辨率中受到限制。我们最近开发了用于映射3D基因组组织的DNase HI-C，其使用DNase I进行染色质碎片。 DNase Hi-C克服了与传统的HI-C方法相关的重新相关的限制，从而提高了方法决议。此外，将该方法与DNA捕获技术组合提供了一种高通量方法（靶向DNA酶HI-C），其允许以极高的分辨率绘制细级染色质架构。因此，靶向的DNase Hi-C对于描绘控制基因表达的CIS-SCOUNT网络的物理景观以及表型相关的染色质3D签名的表型相关的染色质3D签名是有价值的。在这里，我们为这两种方法提供了对方法设计和逐步工作协议的详细描述。 （c）2018年Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号