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首页> 外文期刊>Microbial Pathogenesis >Identification of 60 secreted proteins for Mycoplasma bovis with secretome assay
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Identification of 60 secreted proteins for Mycoplasma bovis with secretome assay

机译：用克己基测定鉴定60个用于支原体BOVIS的分泌蛋白

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Mycoplasma bovis is a risky pathogen mainly responsible for pneumonia and mastitis in cattle. Up to date, its pathogenesis is not clear. Since secreted proteins have a tricky role in M. bovis pathogenesis, this study was designed to systematically reveal M. bovis secretome and potential role in virulence of the pathogen. By using bioinformatics tools, a total of 246 secreted proteins were predicted based on M bovis genome. Among them, 14 were classical, 154 nonclassical and 78 both pathways. Then by using 2-dimensional gel electrophoresis (2-DE) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF- MS), 169 proteins were revealed. Of them, 60 were predicted to be secreted including 3 classical, 43 non-classical, and 14 both classical and non-classical. Further 8 proteins (MbovP0038, MbovP0338, MbovP0341, MbovP0520, MbovP0581, MbovP0674, MbovP0693, MbovP0845) were predicted to be virulence-related factors with VFDB. In addition, MbovP0581 (ABC transporter protein) was validated experimentally as secreted in nature and highly immunogenic reacting with sera of cattle experimentally infected with M. bovis. In conclusion, this study might be a crucial step towards a better understanding of pathogenesis and leading to the development of novel diagnostic marker and potent vaccine against M. bovis.
机译：支原体Bovis是一种风险的病原体，主要负责牛粪和牛粪中的乳腺炎。迄今为止，其发病机制尚不清楚。由于分泌的蛋白质在M. Bovis发病机制中具有棘手的作用，因此该研究旨在系统地揭示植物肠胚和病原体毒力的潜在作用。通过使用生物信息学工具，基于M Bovis基因组预测总共246个分泌的蛋白质。其中，14个是古典的，154个非无论如何和78个途径。然后通过使用二维凝胶电泳（2-DE）和基质辅助激光解吸/电离飞行质谱（MALDI-TOF-MS），揭示了169个蛋白质。其中，预计60待分泌，包括3个经典，43个非古典和14个经典和非古典。此外，预计8例蛋白（MBoVP0038，MBoVP0338，MBoVP0581，MBoVP0674，MBoVP0581，MBoVP0674，MBoVP0693，MBoVP0845）是具有VFDB的毒力相关因素。此外，MBOVP0581（ABC转运蛋白）在实验上被实验验证，如同用M. Bovis实验感染的牛血清的高度免疫原性反应。总之，这项研究可能是更好地理解发病机制并导致对棉花的新型诊断标志物和强效疫苗的发展至关重要的步骤。

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