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Immunological characteristics of Mycobacterium tuberculosis subunit vaccines immunized through different routes

机译：不同途径免疫分枝杆菌结核分枝杆菌的免疫特征

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摘要

Tuberculosis is chronic infectious disease caused by Mycobacterium tuberculosis (M.tb) that is prevalent worldwide. Several specific antigens, such as Antigen 85B (Ag85B) and 6 kDa early secretory antigenic target (ESAT-6) protein of M.tb, are listed as some of the candidate subunit vaccines against M.tb. ESAT-6, as a virulent factor and differential gene in M.tb, shows insufficient immunogenicity in animal model. In order to investigate the ways to improve the immunogenicity of ESAT-6, we immunized ESAT-6 by subcutaneous and intramuscular routes with different adjuvants. We found that ESAT-6 immunized alone did not induce significant humoral immunity in both immunization routes. However, subcutaneous immunization of ESAT-6 plus incomplete Freund's adjuvant can induce a significant humoral immune response, enhanced proliferation and elevated secretion of IFN-gamma from splenocytes. Intramuscular immunization of ESAT-6 plus adjuvant aluminum salt or poly(I:C) did not enhance humoral and cellular immune responses. Therefore, it is concluded that immunization of ESAT-6 subcutaneously plus incomplete Freund's adjuvant induces stronger humoral and cellular immune responses, which can be considered of ESAT-6 as a subunit vaccine in further research against tuberculosis.
机译：结核病是由全球普遍普遍的结核病（M.TB）引起的慢性传染病。诸如M.TB的抗原85b（Ag85b）和6kDa早期分泌物抗原靶（Esat-6）蛋白的几种特异性抗原被列为对M.TB的一些候选亚基疫苗。 ESAT-6作为M.TB中的毒力因子和差异基因，在动物模型中显示出不充分的免疫原性。为了研究改善ESAT-6的免疫原性的方法，我们通过皮下和肌肉内途径免疫ESAT-6与不同的佐剂。我们发现单独免疫的ESAT-6在免疫途径中没有诱导显着的体液免疫力。然而，对ESAT-6加不完全弗氏佐剂的皮下免疫可诱导显着的体液免疫应答，增强来自脾细胞IFN-Gamma的增殖和升高。 Esat-6加上佐剂铝盐或聚（I：C）的肌内免疫并未增强体液和细胞免疫应答。因此，得出结论，ESAT-6皮下免疫加上不完全弗氏佐剂诱导更强的体液和细胞免疫应答，其可考虑ESAT-6作为亚基疫苗进一步研究抗结核疫苗。

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  • 来源
    《Microbial Pathogenesis》 |2018年第2018期|共9页
  • 作者

    Lu Yanzhi; Kang Jian; Ning Huanhuan; Wang Lifei; Xu Yanhui; Xue Ying; Xu Zhikai; Wu Xingan; Bai Yinlan;

  • 作者单位

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

    Fourth Mil Med Univ Dept Microbiol Coll Basic Med Sci 169 Changle West Rd Xian 710032 Shaanxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    ESAT-6; Mycobacterium tuberculosis; Immunization route; Adjuvant;

    机译：ESAT-6;结核分枝杆菌;免疫途径;佐剂;

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