...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advances in pharmacological sciences >Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis
【24h】

Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis

机译：鸭chin虫DC抑制纤维肉瘤细胞系生长和诱导凋亡的体外分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Echinophora platyloba DC plant (Khousharizeh) is one of the indigenous medicinal plants which is used as a food seasoning and medicine in Iran. The objective of this study was to examine the in vitro cytotoxic activity and the mechanism of cell death of crude methanolic extracts prepared from Echinophora platyloba DC, on mouse fibrosarcoma cell line (WEHI-164). Cytotoxicity and viability of methanolic extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoUum bromide (MTT) and dye exclusion assay. Cell death ELISA was employed to quantify the nucleosome production result from nuclear DNA fragmentation during apoptosis and determine whether the mechanism involves induction of apoptosis or necrosis. The cell death was identified as apoptosis using terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP nick end labeling (TUNEL) assay. Our results demonstrated that the extract decreased cell viability, suppressed cell proliferation, and induced cell death in a time- and dose-dependent manner in WEHI-164 cells (IC50 = 196.673 +- 12.4 mug/mL) when compared with a chemotherapeutic anticancer drug, Toxol. Observation proved that apoptosis was the major mechanism of cell death. So the Echinophora platyloba DC extract was found to time- and dose-dependently inhibit the proliferation of fibrosarcoma cell possibly via an apoptosis-dependent pathway.
机译：Echinophora platyloba DC植物（Khousharizeh）是土生土长的药用植物之一，在伊朗用作食品调味料和药品。这项研究的目的是检查从小鼠棘皮肉瘤细胞系（WEHI-164）上提取的Echinophora platyloba DC制备的粗甲醇提取物的体外细胞毒性活性和细胞死亡的机制。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四溴甲烷（MTT）和染料排斥试验评估甲醇提取物的细胞毒性和生存能力。细胞死亡ELISA用于量化凋亡过程中核DNA片段化产生的核小体产生结果，并确定该机制是否涉及诱导凋亡或坏死。使用末端脱氧核苷酸转移酶（TdT-）介导的dUTP缺口末端标记（TUNEL）分析将细胞死亡鉴定为凋亡。我们的结果表明，与化学抗癌药相比，WEHI-164细胞中提取物以时间和剂量依赖性方式降低了细胞活力，抑制了细胞增殖，并诱导了细胞死亡（IC50 = 196.673 +-12.4 mug / mL）。 ，Toxol。观察证明凋亡是细胞死亡的主要机制。因此，发现白屈菜DC提取物可能通过凋亡依赖性途径来时间和剂量依赖性地抑制纤维肉瘤细胞的增殖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号