On the driving forces in the type-II enzymes - Restriction molecular motors

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On the driving forces in the type-II enzymes - Restriction molecular motors

机译：关于II型酶的驱动力-限制性分子马达

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The motion of the type-II enzyme-DNA complex along the DNA chain is described in the framework of the model of chiral kink movement under the action of a transverse electric field of moderate strength and the model of proper kinetic equations. The basic enzyme characteristics are estimated. The energy released during catalytic reactions due to the participation of certain metal-ions is used to produce bending and twisting of the DNA, the angular motion, and the linear movement of the enzyme along the DNA chain. When water molecules and ions are expelled from the protein-DNA interface, a large longitudinal force appears in the motor, which strives to turn the deformed part of the DNA chain around the transverse axis, which can scissor the DNA chain in some "weak" place inside the enzyme groove.

机译：II型酶-DNA复合物沿着DNA链的运动在中等强度的横向电场作用下的手性扭结运动模型和适当的动力学方程模型的框架内描述。估计基本的酶特性。由于某些金属离子的参与，在催化反应过程中释放的能量用于产生DNA的弯曲和扭曲，角运动以及酶沿着DNA链的线性运动。当水分子和离子从蛋白质-DNA界面排出时，马达中会出现很大的纵向力，力会试图使DNA链的变形部分绕横轴转动，从而可以将DNA链剪成“弱”状态置于酶槽内。

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《Crystallography reports》 |2007年第6期|共6页
作者
Pikin SA;
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原文格式 PDF
正文语种 eng
中图分类晶体学;
关键词
DNA; BINDING; ENDONUCLEASES; SUBSTRATE; COMPLEXES; CLEAVAGE; RELEASE; ECORI; SITE;

机译：DNA;结合;核酸内切酶;基质;复合物;切割;释放;ECORI;位点;

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1. On the driving forces in the type-II enzymes - Restriction molecular motors [J] . Pikin SA Crystallography reports . 2007,第6期

机译：关于II型酶的驱动力-限制性分子马达
2. Two different isoschizomers of the type-II restriction endonuclease Taq I (T/CGA) within the same Thermus isolate: Tsp32 I, an enzyme with similar heat stability properties to the prototype enzyme Taq I, and Tsp32 II, a hyperthermostable isoschizomer of Taq I [J] . R A D Williams, S G Welch The biochemical journal . 1995,第2期

机译：同一Thermus分离物中的II型限制性核酸内切酶Taq I（T / CGA）的两种不同的同分异构体：Tsp32 I（一种与原型酶Taq I具有相似的热稳定性质的酶）和Tsp32 II（一种Taq I的超热异构体）
3. On the DNA Cleavage by Restriction Enzymes- Molecular Motors with Polarization Properties [J] . S. A. Pikin Molecular crystals and liquid crystals . 2009,第508期

机译：限制性内切酶对DNA的极化作用
4. A Realization of DNA Molecular Machine That Walks Autonomously by Using a Restriction Enzyme [C] . Hiroyuki Sekiguchi, Ken Komiya, Daisuke Kiga, International Meeting on DNA Computing . 2008

机译：通过使用限制酶来实现DNA分子机的DNA分子机
5. Molecular motor forces and nuclear positioning in living cells [D] . Wu, Jun 2012

机译：分子动力与活细胞中的核定位
6. Two different isoschizomers of the type-II restriction endonuclease Taq I (T/CGA) within the same Thermus isolate: Tsp32 I an enzyme with similar heat stability properties to the prototype enzyme Taq I and Tsp32 II a hyperthermostable isoschizomer of Taq I. [O] . S G Welch, R A Williams 1995

机译：同一Thermus分离物中的II型限制性核酸内切酶Taq I（T / CGA）的两种不同的同分异构体：Tsp32 I（一种具有与原型酶Taq I相似的热稳定性质的酶）和Tsp32 II（一种Taq I的超热异构体）。
7. Two different isoschizomers of the type-II restriction endonuclease Taq I (T/CGA) within the same Thermus isolate: Tsp32 I, an enzyme with similar heat stability properties to the prototype enzyme Taq I, and Tsp32 II, a hyperthermostable isoschizomer of Taq I. [O] . Welch, S G, Williams, R A 1995

机译：同一Thermus分离物中的II型限制性核酸内切酶Taq I（T / CGA）的两种不同的同分异构体：Tsp32 I（一种具有与原型酶Taq I相似的热稳定性质的酶）和Tsp32 II（一种Taq I的超热异构体）。
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机译：制定高能见度执法运动的指导方针，以减少客运和商用机动车驾驶员的不安全驾驶行为：基于票务激进的汽车和卡车（TaCT）试点项目的选择性交通执法计划（sTEp）

On the driving forces in the type-II enzymes - Restriction molecular motors

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