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首页> 外文期刊>Molecular biology reports >The efficiency of Cytochrome P450 gene-based markers in accessing genetic variability of drumstick (Moringa oleifera Lam.) accessions
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The efficiency of Cytochrome P450 gene-based markers in accessing genetic variability of drumstick (Moringa oleifera Lam.) accessions

机译：基于细胞色素P450基因基因的效率在鼓槌遗传变异中的遗传变异性（Moringa Oleifera Lam。）

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摘要

Drumstick (Moringa oleifera Lam.) is an important vegetable as well as forage crop of arid and semi-arid zones of the tropics. The leaves and pods of the plant are rich sources of minerals and vitamins. In the present work, genetic diversity study of 23 genotypes of M. oleifera collected from Kerala, Tamil Nadu and Karnataka states of India was carried out using seven cytochrome P450 (CytP450) markers. By using seven pairs of CytP450 gene-based markers, 88.25% of polymorphism was recorded among the 23 sampled genotypes. The Polymorphic Information Content (PI), Marker Index (MI) and Resolving Power obtained for seven primers were estimated 0.23, 2.96 and 9.83, respectively. The Unweighted Pair Group Method with Arithmetic mean (UPGMA) dendrogram based on this marker data indicate that genotypes from different geographical regions are placed in the same clusters. The dendrogram and Principal Coordinates Analysis (PCoA) plots derived from the binary data matrices were highly concordant. The investigation, in brief, proved that CytP450 based marker system is efficient in the elucidation of genetic diversity in M. oleifera accessions.
机译：鼓槌（Moringa Oleifera Lam。）是一种重要的蔬菜，以及热带地区的干旱和半干旱区的饲养作物。该植物的叶子和荚是丰富的矿物质和维生素。在目前的工作中，使用七种细胞色素P450（Cytp450）标记进行了从喀拉拉邦，泰米尔纳德邦和印度喀拉塔卡州的23种基因型的遗传多样性研究。通过使用七对CyTP450基因基本标记，在23种取样基因型中记录了88.25％的多态性。多态性信息含量（PI），标记指数（MI）和7个引物获得的分辨率分别估计为0.23,2.96和9.83。基于该标记数据的算术平均值（UPGMA）树木的未加权对组方法表明来自不同地理区域的基因型被放置在同一簇中。从二进制数据矩阵导出的树形图和主要坐标分析（PCOA）块的曲线是高度一致的。简而言之，调查证明，基于CYTP450的标记体系在M. Oleifera释放遗传多样性阐明中是有效的。

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