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首页> 外文期刊>Mycoses: Diagnosis, therapy and prophylaxis of fungal diseases >Identification and molecular epidemiology of dermatophyte isolates by repetitive‐sequence‐ PCR PCR ‐based DNA DNA fingerprinting using the DiversiLab system in Turkey
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Identification and molecular epidemiology of dermatophyte isolates by repetitive‐sequence‐ PCR PCR ‐based DNA DNA fingerprinting using the DiversiLab system in Turkey

机译：通过土耳其多因素系统的重复序列 - PCR PCR基于DNA DNA指纹识别和分子流行物分离物的鉴定和分子流行病学

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Summary Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence‐based PCR (rep‐PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep‐PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep‐PCR. In the identification of dermatophyte isolates, agreement between rep‐PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A ‐D) which were determined by the use of rep‐PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep‐PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep‐PCR.
机译：发明内容临床样本中的Dermatophyte物种，分离和鉴定仍然困难，需要很长时间。通过将结果与参考鉴定的结果进行比较，评估通过重复序列的PCR（REP-PCR）在土耳其临床实验室中常量分离的皮肤病的鉴定和分子流行病学。研究了在坎默里和24例哈及患者中，共有44例从20例肤色骨膜患者中分离的44例皮肤病。皮肤阴影分离株的鉴定基于使用多因素系统（BioMerieux）的参考鉴定和Rep-PCR。来自不同患者的Dermatophyte分离株的基因分型通过Rep-PCR测定。在鉴定皮肤阴影分离株中，REP-PCR与常规方法之间的一致性为87.8％（36个，共41个）。 Dermatophyte菌株属于通过使用Rep-PCR来确定的四个克隆（A -D）。克隆B，D中的皮肤病菌株显示出相同的图案相对于该区域。总之，REP-PCR似乎有助于评估滴体鲁姆物种复合物和滴岩术术术复合物分离株的鉴定和克隆关系。这些分离株的相似性和多样性可以根据REP-PCR的不同区域进行评估。

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