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首页> 外文期刊>Molecular reproduction and development >Sperm motility initiating substance may be insufficient to induce forward motility of Cynops ensicauda sperm
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Sperm motility initiating substance may be insufficient to induce forward motility of Cynops ensicauda sperm

机译：精子运动引发物质可能不足以诱导Cynops Ensicauda精子的前向动力

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摘要

Sperm motility-initiating substance (SMIS) is a key protein for internal fertilization of the newt, Cynops pyrrhogaster, and commonly enhances forward sperm motility in some amphibian species, including external fertilizers. SMIS action varies among different species in correlation with a species-specific reproductive environment. In the present study, we identified the gene of C. ensicauda SMIS (CeSMIS) and examined the mechanism of SMIS action with reference to that of the closely related Cynops species. The CeSMIS was identified by a 176-amino acid sequence including seven amino acids critical for the initiation of sperm motility. The amino acid sequence showed 91% homology to the whole sequence of C. pyrrhogaster SMIS (CpSMIS). By immunostaining with an anti-CpSMIS antibody, CeSMIS was shown to be localized in the outer layer of the egg jelly. A peptide presenting the active site of SMIS was observed to bind to the axial rod of the midpiece in C. ensicauda sperm. The localization and binding patterns of CeSMIS were fundamentally similar to those of CpSMIS. However, the SMIS peptide did not induce forward motility of C. ensicauda sperm, although it induced a fast wave of the undulating membrane. Forward sperm motility was induced in the egg jelly extract containing CeSMIS. These results suggest that the mechanism of initiation of sperm motility is differentiated between C. ensicauda and C. pyrrhogaster.
机译：精子运动引发物质（SMIS）是蝾螈，Cynops Pyrrhogaster的内部受精的关键蛋白，并且通常提高一些两栖物种的精子运动，包括外部肥料。 SMIS行动在不同物种中不同于物种特异性生殖环境。在本研究中，我们鉴定了C.SeNicauda Smis（Cesmis）的基因，并参考密切相关的Cynops物种的SMIS行动机制。通过176-氨基酸序列鉴定Cesmis，其包括七个氨基酸对于开始精子活性至关重要。氨基酸序列显示出91％的同源性与整个C.Pyrrhogaster Smis（CPSMI）的同源性。通过用抗CPSMIS抗体免疫染色，显示CESMIS在蛋果冻的外层中定位。观察到呈现SMIS活性位点的肽，以与C.Senicauda精子中的中间件的轴向杆结合。 Cesmis的定位和结合模式从根本上类似于CPSMIS。然而，SMIS肽没有诱导C. Ensicauda精子的前正常运动，尽管它诱导了起伏膜的快速波。在含有Cesmis的蛋果冻萃取物中诱导转发精子活力。这些结果表明，在C.Senicauda和C.Pyrrhogaster之间的分化了精子运动的启动机制。

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