Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

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Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

机译：基于酶的细胞术，一种敏感的单细胞cytric方法，用于评估CML中的BCR-ABL1活性

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We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

机译：我们开发了一种简单，快速且经济高效的酶促细胞术平台，以测量细胞内信号通路活性。我们的单细胞微孔阵列平台使用酶促信号扩增和利用Michaelis-Menten动力学量化蛋白质磷酸化。与常规流式细胞术相比，我们的方法在分辨率上提供了两倍的增加。

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《Lab on a chip》 |2020年第5期|共7页
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正文语种 eng
中图分类化学实验（实验化学）;生物化学;生物科学;化学;
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1. Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML [J] . Lab on a chip . 2020,第5期

机译：基于酶的细胞术，一种敏感的单细胞cytric方法，用于评估CML中的BCR-ABL1活性
2. DNA Analysis of Mutations in the Kinase Domain of BCR-ABL1 By Allele-Specific Digital PCR Is Highly Sensitive and Refines Prediction of Kinetics of Resistant CML Clones [J] . Polivkova Vaclava, Curik Nikola, Zizkova Hana, Blood: The Journal of the American Society of Hematology . 2018,第NovaelaTreatmentAppr期

机译：等位基因特异性数字PCR的BCR-ABL1激酶结构域中的突变DNA分析是高敏感的，精制的抗性CML克隆动力学预测
3. Clinical Validation of a Highly Sensitive and Highly Reproducible BCR-ABL1 Quantification Assay for CML Monitoring [J] . Alikhan M., Lu P., Wang Y. The Journal of molecular diagnostics: JMD . 2017,第6期

机译：用于CML监测的高度敏感和高度可重复的BCR-ABCR1定量测定的临床验证
4. Assessing the Ability of Image Processing Methods of Droplets Sprayed on Water Sensitive Papers for Aerial Application [C] . Gang Xu, Ruirui Zhang, Liping Chen, IFIP WG International Conference on Computer and Computing Technologies in Agricultur . 2019

机译：评估喷涂在水敏纸上喷射的液滴图像处理方法的能力
5. Investigations of T cell costimulation and autoimmunity in mice, and development of flow cytometric methods to assess lymphocyte stimulation in dogs. [D] . Weatherill, Amy R. 2002

机译：小鼠T细胞共刺激和自身免疫的研究，以及流式细胞术方法在评估狗淋巴细胞刺激中的发展。
6. Flow Cytometry-Based Methods for Assessing Soluble scFv Activities and Detecting Antigens in Solution [O] . Sean A. Gray, Kris M. Weigel, Keith D. Miller, -1

机译：基于流式细胞术评估可溶性SCFV活性和溶液中抗原的方法
7. BCR-ABL1 doubling times more reliably assess the dynamics of CML relapse compared with the BCR-ABL1 fold rise: implications for monitoring and management [O] . Branford, S., Yeung, D., Prime, J., 2012

机译：与BCR-aBL1倍数上升相比，BCR-aBL1倍增时间更可靠地评估CmL复发的动态：对监测和管理的影响

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

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