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首页> 外文期刊>LWT-Food Science & Technology >Diversity of isolated lactic acid bacteria in Ya'an sourdoughs and evaluation of their exopolysaccharide production characteristics
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Diversity of isolated lactic acid bacteria in Ya'an sourdoughs and evaluation of their exopolysaccharide production characteristics

机译:雅安酵母菌分离乳酸菌的多样性及其外糖生产特征评价

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摘要

The lactic acid bacterial diversity of wheat sourdoughs collected in Ya'an city, Sichuan province was investigated by culture-dependent methods combined with 16S rRNA gene sequencing, and the exopolysaccharide (EPS) production characteristics of ropy lactic acid bacteria (LAB) strains from these sourdoughs were evaluated. Two hundred nineteen LAB strains were isolated, and genotypic characterization indicated that the isolated LAB strains included Lactobacillus plantarum (L plantarum), L. pantheris, Leuconostoc citretan (Leu. citreum), Weissella viridescens, Leu pseudomesenteroides, Lactococcus lactis (L. lactis), L. raffinolactis, and Leu. mesenteroides. All the isolates were found to harbor genes coding for EPS, and the yield of EPS produced by "ropy" LAB strains ranged between 2.3 and 229.5 mg/g wet cell weight. The present study enhances our knowledge about LAB diversity in Ya'an sourdoughs and provides information about the EPS-producing LAB strains in sourdough samples, which might be favorable for the application of specific LAB strains in sourdough technology.
机译:四川省雅安市收集的小麦酵母乳酸菌多样性,依赖于培养依赖性方法研究了16S rRNA基因测序,以及来自这些的r型乳酸菌(实验室)菌株的外糖(EPS)生产特征评估酵母。分离两百九九实验室菌株,基因型表征表明,该孤立的实验室菌株包括乳酸杆菌(L Flatraum),L.Pantheris,Leuconostoc Citretan(Leu。Citreum),Weissella Viridescens,Leu Pseudomesenteroides,乳酸乳球菌(L. Lactis) ,L. raffinolactis和Leu。肠系膜。发现所有分离株均为编码EPS编码的基因,并“Ropy”实验室菌株产生的EPS的产量范围为2.3和229.5mg / g湿细胞重量。本研究提高了我对雅安酵母的实验多样性知识,并提供了有关酸谷样品中的产卵实验室菌株的信息,这可能有利于在酵母技术中应用特定的实验室菌株。

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