Diversity of anaerobic arsenite-oxidizing bacteria in low-salt environments analyzed with a newly developed PCR-based method

Ospino Melody Cabrera; Kojima Hisaya; Watanabe Tomohiro; Iwata Tomoya; Fukui Manabu

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Diversity of anaerobic arsenite-oxidizing bacteria in low-salt environments analyzed with a newly developed PCR-based method

机译：用新开发的基于PCR的方法分析低盐环境中厌氧砷酸盐氧化细菌的多样性

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Anaerobic arsenite oxidation is potentially important but the least understood process in the arsenic cycle. The catalytic subunit of the key enzyme for anaerobic arsenite oxidation is encoded by the arxA gene. In this study, a novel primer pair for the arxA gene was designed to detect diverse sequences of this notable gene. Further modification of the designed primer was made by adding extra bases to its 5'- end. This modification made it possible to analyze the PCR products with TA cloning, which provides higher throughput of investigations. With the combination of modified primer pair and TA cloning, diverse arxA gene sequences were effectively obtained from samples of lake water, spring water, and hot spring microbial mat. The sequences detected in the samples characterized by low salinity and nearly neutral pH were phylogenetically distinct from the majority of previously known arxA genes, found in the genome of alkaliphiles and halophiles.

机译：Anaerobic砷酸氧化可能是重要的，但最不理解的砷循环中的过程。厌氧亚砷酸盐氧化的关键酶的催化亚基由ARXA基因编码。在该研究中，设计用于ARXA基因的新型引物对以检测该值得注意基因的不同序列。通过将额外的碱添加到其5'末端来进行设计的引物的进一步改变。这种改进使得可以使用Ta克隆分析PCR产品，这提供了更高的研究吞吐量。随着改性引物对和Ta克隆的组合，各种ARXA基因序列有效地从湖水，泉水和热弹簧微生物垫的样品中获得。在盐度低盐度和几乎中性pH的样品中检测到的序列是从碱和卤素基因组中发现的大多数先前已知的ARXA基因。

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来源
《Limnology》 |2018年第2期|共7页
作者
Ospino Melody Cabrera; Kojima Hisaya; Watanabe Tomohiro; Iwata Tomoya; Fukui Manabu;
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作者单位

Hokkaido Univ Inst Low Temp Sci Kita Ku Kita 19 Nishi 8 Sapporo Hokkaido 0600819 Japan;

Hokkaido Univ Inst Low Temp Sci Kita Ku Kita 19 Nishi 8 Sapporo Hokkaido 0600819 Japan;

Hokkaido Univ Inst Low Temp Sci Kita Ku Kita 19 Nishi 8 Sapporo Hokkaido 0600819 Japan;

Univ Yamanashi Dept Environm Sci Kofu Yamanashi Japan;

Hokkaido Univ Inst Low Temp Sci Kita Ku Kita 19 Nishi 8 Sapporo Hokkaido 0600819 Japan;

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原文格式 PDF
正文语种 eng
中图分类湖泊、水库;淡水生物;
关键词
Arsenic; Arsenite oxidase; arxA; PCR detection; Primer design;

机译：砷;砷酸氧化酶;ARXA;PCR检测;底漆设计;

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专利

1. Diversity of anaerobic arsenite-oxidizing bacteria in low-salt environments analyzed with a newly developed PCR-based method [J] . Ospino Melody Cabrera, Kojima Hisaya, Watanabe Tomohiro, Limnology . 2018,第2期

机译：用新开发的基于PCR的方法分析低盐环境中厌氧砷酸盐氧化细菌的多样性
2. Effect of Antibiotics on Redox Transformations of Arsenic and Diversity of Arsenite-Oxidizing Bacteria in Sediment Microbial Communities [J] . Shigeki Yamamura, Keiji Watanabe, Wataru Suda, Environmental Science & Technology . 2014,第1期

机译：抗生素对沉积物微生物群落中砷氧化还原转化及亚砷氧化细菌多样性的影响
3. Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan [J] . Suvendu Das, Sandeep Kar, Jiin-Shuh Jean, Water Research . 2013,第19期

机译：台湾西南部黑脚病流行地区北门市地下水中深度分解的砷氧化细菌的丰富度和多样性
4. Optimization of Producing Enzyme Conditions for Facultative Anaerobic Cellulose Degrading Bacteria in Aerobic and Anaerobic Environments [C] . Ting-ting Fan, Si-ying Liu, Cheng-wen Wang, Mechanical engineering and technology . 2011

机译：有氧和厌氧环境中兼性厌氧纤维素降解菌生产酶条件的优化
5. Developing a biosensor for the detection of bacteria: A comparison of methods for isolating bacteria-specific antibodies. [D] . Walper, Scott Allen. 2010

机译：开发用于检测细菌的生物传感器：分离细菌特异性抗体的方法比较。
6. Diversity of Culturable Psychrophilic and Psychrotrophic Anaerobic Bacteria Isolated from Beef Abattoirs and Their Environments [O] . G. Moschonas, D. J. Bolton, D. A. McDowell, 2011

机译：牛肉屠宰场及其周围环境中可培养的嗜冷和厌氧厌氧菌的多样性
7. Unsuspected Diversity of Arsenite-Oxidizing Bacteria as Revealed by Widespread Distribution of the aoxB Gene in Prokaryotes ▿ † [O] . Heinrich-Salmeron, Audrey, Cordi, Audrey, Brochier-Armanet, Céline, 2011

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8. Phylogenetic and Metabolic Diversity of Hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX)-transforming Bacteria in Strictly Anaerobic Mixed Cultures Enriched on RDX as Nitrogen Source. [R] . Zhao, J., Spain, J., Hawari, J. 2003

机译：六氢-1,3,5-三硝基-1,3,5-三嗪（RDX）转化细菌的系统发育和代谢多样性在严格厌氧混合培养物中富集在RDX上作为氮源。

Diversity of anaerobic arsenite-oxidizing bacteria in low-salt environments analyzed with a newly developed PCR-based method

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