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首页> 外文期刊>Results and problems in cell differentiation >Chapter 11 Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes
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Chapter 11 Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes

机译：第11章染色质改造酶和组蛋白转录物的累积牛卵母细胞

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During growth, the oocyte accumulates mRNAs that will be required in the later stages of oogenesis and early embryogenesis until the activation of the embryonic genome. Each of these developmental stages is controlled by multiple regulatory mechanisms that ensure proper protein production. Thus mRNAs are stabilized, stored, recruited, polyadenylated, translated and/or degraded over a period of several days. As a consequence, understanding the biological significance of changes in the abundance of transcripts during oocyte growth and differentiation is rather complex. Nevertheless the availability of transcriptomic platforms applicable to scarce samples such as oocytes has generated large amounts of data that depict the transcriptome of oocytes under different conditions. Despite several technical constrains related to protein determination in oocytes that still limit the possibility to verify certain hypothesis, it is now possible to use mRNA levels to start building plausible scenarios. To start deciphering the changes in the level of specific mRNAs involved in chromatin remodelling, we have performed a meta-analysis of existing microarray datasets from germinal vesicle (GV) stage bovine oocytes during the final stages of oocyte differentiation. We then analysed the expression profiles of histone and histone-remodelling enzyme mRNAs and correlated these with the major histone modifications known to occur at the same period, based on data available in the literature. We believe that this approach could reveal the function of specific enzymes in the oocyte. In turn, this information will be useful in future studies, which final ambitious goal is to decipher the 'oocyte-specific histone code'.
机译：在生长期间，卵母细胞累积了在胚芽内和早期胚胎发生的后期阶段需要的MRNA，直至激活胚胎基因组。这些发展阶段中的每一个由多种调节机制控制，确保适当的蛋白质产生。因此，在几天的时间内稳定，储存，募集，多腺苷酸化，翻译和/或降解。因此，了解卵母细胞生长和分化期间转录物丰富变化的生物学意义相当复杂。然而，适用于缺乏卵母细胞的稀缺样本的转录组平台的可用性产生了大量的数据，其描绘了不同条件下的卵母细胞的转录组。尽管卵母细胞中蛋白质测定有几次技术约束，但仍然限制了验证某些假设的可能性，现在可以使用MRNA水平来开始建立合理的情景。为了开始解密染色蛋白重塑所涉及的特异性mRNA水平的变化，我们在卵母细胞分化的最终阶段期间从生发囊泡（GV）阶段牛卵母细胞中的现有微阵列数据集进行了荟萃分析。然后，我们分析了组蛋白和组蛋白重塑酶MRNA的表达谱并基于文献中可用的数据在同一时期发生的主要组蛋白修饰相关。我们认为这种方法可以揭示卵母细胞中特定酶的功能。反过来，这些信息对于未来的研究将有用，最终的雄心勃勃的目标是破译“特定oOcyte的组蛋白代码”。

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