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首页> 外文期刊>Reproductive sciences >Properties of Epithelial Cells and Vaginal Secretions in Pregnant Women When Lactobacillus crispatus or Lactobacillus iners Dominate the Vaginal Microbiome
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Properties of Epithelial Cells and Vaginal Secretions in Pregnant Women When Lactobacillus crispatus or Lactobacillus iners Dominate the Vaginal Microbiome

机译：当乳酸杆菌或乳酸杆菌中的孕妇上皮细胞和阴道分泌物的性质主导阴道微生物组

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Objective: Our objective was to determine differences in properties of vaginal epithelial cells and the composition of vaginal secretions when Lactobacillus crispatus or Lactobacillus iners are numerically dominant in the vaginal microenvironment of pregnant women. Methods: The vaginal microbiomes of 157 first-trimester pregnant women were identified by classifying partial 16S gene sequences amplified from the V1 to V3 region of bacterial ribosomal 16S RNA genes. The extent of autophagy and cell stress in vaginal epithelial cells was determined by measuring the intracellular levels of p62 and the inducible 70-kDa heat shock protein (hsp70). Vaginal secretions were analyzed using a colorimetric assay for d - and l -lactic acid and by enzyme-linked immunosorbent assay for matrix metalloproteinase 8, neutrophil gelatinase-associated lipocalin, α-amylase, hyaluronan, calprotectin, S100A8, and extracellular matrix metalloproteinase inducer (EMMPRIN). Results: L. crispatus was dominant in 69 (43.9%) women, while L iners dominated in 23 (14.6%) women. The median epithelial p62 levels were 0.41 and 4.26 ng/mL in women with L crispatus or L iners dominance, respectively ( P = .0035). The corresponding median hsp70 levels were 4.24 and 14.50 ng/mL, respectively ( P < .0001). The d -lactic acid concentration in vaginal fluid was highest in association with L crispatus dominance, while all other vaginal fluid compounds except for EMMPRIN were highest when L iners was dominant ( P < .03). Conclusion: Epithelial cells exhibit a higher level of autophagy, lower induction of stress-related hsp70, and release lower level of mediators when L crispatus is most abundant as compared to when L iners dominates the vaginal microbiota.
机译：目的：我们的目的是确定阴道上皮细胞和阴道分泌物组成的差异，当乳酸杆菌或乳酸杆菌在孕妇的阴道微环境中数量占主导地位时。方法：通过对从V1至V3区域的细菌核糖体16S RNA基因的v1至V3区域扩增的部分16s基因序列来鉴定157个孕孕孕妇的阴道微生物。通过测量p62的细胞内水平和诱导型70-kda热休克蛋白（Hsp70）来确定阴道上皮细胞中自噬和细胞应激程度。使用比色测定法分析阴道分泌物，用于D - 和L-乳酸和基质金属蛋白酶8，中性粒细胞明胶酶相关的脂素素，α-淀粉酶，透明质酸，CalProtectin，S100a8和细胞外基质金属蛋白酶诱导剂（ Emmprin）。结果：L. Crispatus在69名（43.9％）妇女中占主导地位，而L. INER在23名（14.6％）女性中占主导地位。中位上皮P62水平分别为LISPATUS或LINERS占优势的女性中的0.41和4.26ng / ml（p = .0035）。相应的中值Hsp70水平分别为4.24和14.50ng / ml（p <.0001）。阴道液中的D-乳酸浓度与L Cresspatus的优势相关，而除emmprin之外的所有其他阴道流体化合物最高，当L Iners占优势时（p <.03）。结论：上皮细胞表现出较高水平的自噬，较低诱导应力相关的HSP70，并且当L薄脆肌如L Iners主导阴道微生物群时，当L Crisspatus最丰富时，释放较低水平的介质。

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