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首页> 外文期刊>Oncology Research >MicroRNA-18a Targets IRF2 and CBX7 to Promote Cell Proliferation in Hepatocellular Carcinoma
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MicroRNA-18a Targets IRF2 and CBX7 to Promote Cell Proliferation in Hepatocellular Carcinoma

机译：microRNA-18A靶向IRF2和CBX7，以促进肝细胞癌中的细胞增殖

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摘要

MicroRNAs (miRNAs) are a family of noncoding RNAs of similar to 22 nt in length that play important roles in the tumor initiation and progression processes. The aberrant expression status of miR-18a has been reported in hepatocellular carcinoma (HCC). However, the biological role and the underlying mechanism of miR-18a in HCC progression are still to be elucidated. In this study, we examined the expression level of miR-18a in HCC cell lines using the quantitative real-time PCR method. We showed that miR-18a expression in human HCC cell lines was elevated compared with the normal liver cell line. Meanwhile, increasing miR-18a expression by an miR-18a mimic in HCC cell lines promoted cell proliferation and migration, while inhibiting miR-18a expression by an miR-18a inhibitor caused the opposite effects. Using the bioinformatic analyses method, we found that the 3'-untranslated regions (3'-UTRs) of interferon regulatory factor 2 (IRF2) and chromobox protein homolog 7 (CBX7) contain putative binding sequences for miR-18a. Further, luciferase assay validated that both IRF2 and CBX7 were the direct targets of miR-18a in HCC. Moreover, we revealed that overexpression of IRF2 and CBX7 has similar effects as miR-18a downregulation on HCC cell lines. Our results illustrated that miR-18a plays a positive role in HCC progression process by stimulating cell proliferation and migration partly through regulating IRF2 and CBX7.
机译：MicroRNA（miRNA）是一个非编码RNA的家族，其长度与22nt相似，在肿瘤起始和进展过程中起重要作用。肝细胞癌（HCC）报道了miR-18a的异常表达状态。然而，仍阐明MIR-18A在HCC进展中的生物学作用和潜在机制。在这项研究中，我们使用定量实时PCR方法检查了HCC细胞系中miR-18a的表达水平。我们表明，与正常肝细胞系相比，人HCC细胞系中的miR-18a表达升高。同时，在HCC细胞系中模拟MIR-18A模拟的MIR-18A表达促进了细胞增殖和迁移，同时抑制MIR-18A抑制剂的miR-18a表达导致相反的效果。使用生物信息分析方法，我们发现干扰素调节因子2（IRF2）和ChromoBox蛋白质同源物7（CBX7）的3'-未翻译区域（3'-UTRS）含有用于miR-18a的推定结合序列。此外，荧光素酶测定验证了IRF2和CBX7是HCC中miR-18a的直接靶标。此外，我们揭示了IRF2和CBX7的过度表达在HCC细胞系上具有与miR-18a下调的类似效果。我们的结果表明，MIR-18A通过刺激细胞增殖和通过调节IRF2和CBX7来刺激细胞增殖和迁移，在HCC进展过程中发挥积极作用。

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