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首页> 外文期刊>Scientia horticulturae >Analysis of differentially accumulated proteins associated with graft union formation in pecan (Carya illinoensis)
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Analysis of differentially accumulated proteins associated with graft union formation in pecan (Carya illinoensis)

机译：山核桃（Carya Inlinoensis）与移植蛋白形成相关的差分累积蛋白质分析

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摘要

Grafting is of particular importance for the mass cultivation of pecan (Carla illinoensis), which can greatly shorten the long juvenile phase of this species. To reveal the molecular mechanism underlying graft union formation, the dynamic proteome changes at the graft union were investigated 0, 3, 8, 15, and 30 days after grafting in homograft of pecan by two-dimensional gel electrophoresis. Forty-nine protein spots that showed more than two-fold difference in their values were positively identified by mass spectrometry. These identified proteins were associated with energy metabolism, stress and defense responses, protein metabolism, amino acid and nucleotide metabolism, secondary metabolism, hormone related, cell cytoskeleton, and function unknown. Among the proteins, ascorbate peroxidase and peroxiredoxin could scavenge the excess reactive oxygen species induced by wounding, which might play an important role in the graft process. 1-aminocyclopropane-1-car-boxylic acid oxidase probably led to ethylene biosynthesis at the graft interface, participating in the wound response during the early stage of grafting. 30S ribosomal protein seemed to support callus proliferation through promoting the synthesis of proteins. Alpha tubulin and metacaspase might be involved in the differentiation of tracheary elements during graft-union formation. Analysis of the identified proteins will provide insights into the molecular mechanism of graft union formation.
机译：嫁接对山核桃（Carla Intinoensis）的大规模培养特别重要，这可以大大缩短该物种的长幼年阶段。为了揭示覆盖物联合形成的分子机制，通过二维凝胶电泳在悬浮液的同种植物移植物嫁接后，研究了移植愈合的动态蛋白质组变化。通过质谱法正鉴定出它们的值的四十九个蛋白质斑点，其值呈正鉴定。这些鉴定的蛋白质与能量代谢，应激和防御反应，蛋白质代谢，氨基酸和核苷酸代谢，次生代谢，相关，细胞骨骼骨骼和功能未知。在蛋白质中，抗坏血酸过氧化物酶和过洛昔洛辛可以清除伤口诱导的过量的反应性氧物种，这可能在接枝过程中起重要作用。 1-氨基环丙烷-1-轿车蛋白酸氧化酶可能导致移植界面的乙烯生物合成，参与接枝的早期阶段的伤口反应。通过促进蛋白质的合成，30S核糖体蛋白似乎支持愈伤组织增殖。 α管蛋白和Metacaspase可能参与移植工具形成过程中气管元素的分化。对所鉴定的蛋白质的分析将为移植蛋白形成的分子机制提供见解。

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  • 来源
    《Scientia horticulturae》 |2017年第2017期|共9页
  • 作者

    Mo Zhenghai; He Haiyang; Su Wenchuan; Peng Fangren;

  • 作者单位

    Nanjing Forestry Univ Coll Forestry Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Forestry Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Forestry Nanjing 210037 Jiangsu Peoples R China;

    Nanjing Forestry Univ Coll Forestry Nanjing 210037 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Grafting; Pecan; Proteomics; Graft union; Histology;

    机译：嫁接;山核桃;蛋白质组学;移植联盟;组织学;

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