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首页> 外文期刊>Scientia horticulturae >Peach fruit ripening: Proteomic comparative analyses of two cultivars with different flesh texture phenotypes at two ripening stages
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Peach fruit ripening: Proteomic comparative analyses of two cultivars with different flesh texture phenotypes at two ripening stages

机译：桃果实成熟：两种成熟阶段不同肉纹纹理表型的两种品种蛋白质组学对比分析

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摘要

Firmness is an important textural attribute of peach fruit that directly influences its shelf life and consumer acceptance. To investigate the underlying mechanism that determines the texture of peach flesh, we conducted a proteome study on the fruits of two cultivars: hard melting flesh Xiahui 8 and stony hard Xiacui. Then we compared these at the unripe and ripe stages using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In total, 190 proteins with more than 1.5-fold (P < 0.05) abundance changes were observed, including 57 proteins in Xiacui and 171 proteins in Xiahui 8. The differentially expressed proteins in Xiahui 8 were mainly in classes of proteins involved in cellular activities such as sugar metabolism, membrane structure, and cell-cycle control. Our findings imply that the enzymes polygalacturonase (PG), pectate lyase (PL), calmodulin (CaM), calcineurin B-like protein (CBL), peptidyl-prolyl isomerase (PPIase), and 9-cis-epoxycarotenoid dioxygenase (NCED) play functional roles in controlling fruit development and maintaining textural integrity and fruit quality during ripening. The results shed new light on proteomic differences and protein interactions during the metabolism of ethylene, amino acids, sugars, and carbohydrates; the general defense response in cultivars with varied texture phenotypes; and the underlying mechanisms of-peach fruit ripening. They also provide a knowledge base needed for postharvest quality control and breeding strategies for cultivar improvement.
机译：坚定性是桃树的重要态度，直接影响其保质期和消费者的验收。为了研究确定桃子肉体质地的潜在机制，我们对两种品种的果实进行了蛋白质组研究：硬熔肉xiahui 8和石石硬xiacui。然后，我们使用用于亲属和绝对量化（ITRAQ）技术的等值标签在未成熟和成熟阶段进行比较这些。总共观察到具有超过1.5倍（P <0.05）丰度变化的190个蛋白质，其中XIAHUI中的57个蛋白质和Xiahui中的171个蛋白质。夏湖8的差异表达蛋白质主要是在涉及细胞活性的蛋白质中如糖代谢，膜结构和细胞循环控制。我们的研究结果意味着酶聚氨酯酶（PG），果胶酶（PL），钙调蛋白（CAM），钙调解素B样蛋白（CBL），肽基 - 脯氨酰异构酶（PPIASE）和9-CIS-环氧基甲酸二恶英酶（NCED）的比赛在熟化过程中控制水果开发和维持纹理完整性和水果质量的功能作用。结果在乙烯，氨基酸，糖和碳水化合物代谢期间阐明了蛋白质组学差异和蛋白质相互作用的新光;纹理表型种类栽培品种的一般防御反应;和桃果实成熟的潜在机制。他们还提供了采访质量控制和品种改善育种策略所需的知识库。

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  • 来源
    《Scientia horticulturae》 |2020年第2020期|共9页
  • 作者

    Jiang Li; Feng Li; Zhang Fei; Luo Haibo; Yu Zhifang;

  • 作者单位

    Nanjing Agr Univ Coll Food Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Food Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Food Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Normal Univ Sch Food Sci &

    Pharmaceut Engn Nanjing 210023 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Food Sci &

    Technol Nanjing 210095 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Peach fruit; (Non) melting flesh; Ripening; iTRAQ; Proteomic analysis; Prunus persica;

    机译：桃子果;（非）熔化肉;成熟;ITRAQ;蛋白质组学分析;Prunus Persica;

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