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首页> 外文期刊>Sports health >Platelet-Rich Plasma Promotes Migration, Proliferation, and the Gene Expression of Scleraxis and Vascular Endothelial Growth Factor in Paratenon-Derived Cells In Vitro
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Platelet-Rich Plasma Promotes Migration, Proliferation, and the Gene Expression of Scleraxis and Vascular Endothelial Growth Factor in Paratenon-Derived Cells In Vitro

机译：富含血小板血浆的血小板促进在体外甲状腺衍生细胞中患者的迁移，增殖和血管内皮生长因子的迁移，增殖和基因表达

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Background: Platelet-rich plasma (PRP) is a treatment option for tendon injury because of its effective tendon-healing properties. At the early stage of tendon repair, paratenon-derived cells (PDCs) are thought to play a more important role than tendon proper–derived cells (TDCs). However, there has been no study investigating the effects of PRP on PDCs. Hypothesis: PRP promotes the migration, proliferation, and differentiation of PDCs in vitro. Study Design: Controlled laboratory study. Methods: TDCs and PDCs were isolated from the tendon proper and paratenon of rat Achilles tendons and were cultured to the third passage. PRP was prepared from the rats using the double-spin method. Third-passage TDCs and PDCs were cultured in Dulbecco’s modified Eagle medium with 2% fetal bovine serum (control group) or 2% fetal bovine serum plus 5% PRP (PRP group), and cell migration, proliferation, and differentiation were evaluated. The relative mRNA expression levels of scleraxis (Scx), tenomodulin (Tnmd), collagen type I alpha 1 (Col1a1), collagen type III alpha 1 (Col3a1), and vascular endothelial growth factor A (VEGF) were examined by quantitative real-time reverse transcription polymerase chain reaction. Results: The cell migration rate was significantly higher in the PDCs of the PRP group than in the control group (1.4-fold increase; P = 0.02). Cell proliferation was significantly higher in the PDCs of the PRP group (2.2-fold increase; P < 0.01). In the PDCs, the gene expression levels of Scx, Col1a1, and VEGF were significantly increased by PRP (Scx: 2.0-fold increase, P = 0.01; Col1a1: 5.3-fold increase, P = 0.01; VEGF: 7.8-fold increase, P = 0.01), but the gene expression level of Tnmd, a factor for tendon maturation, was significantly reduced by PRP (0.11-fold decrease; P = 0.02). Conclusion: In vitro PRP promoted migration, proliferation, and tenogenic differentiation with the upregulation of Scx in PDCs. PRP also upregulated the expression of the angiogenic marker VEGF. Clinical Relevance: Our results suggest that PRP treatment in vitro may enhance the tendon-healing properties of PDCs at the initial stage of tendon repair.
机译：背景：富含血小板的血浆（PRP）是肌腱损伤的治疗选择，因为其有效的肌腱愈合性能。在肌腱修复的早期阶段，甲状腺衍生的细胞（PDC）被认为比肌腱适当衍生的细胞（TDC）发挥更重要的作用。然而，没有研究PRP对PDC的影响。假设：PRP在体外促进PDC的迁移，增殖和分化。研究设计：受控实验室研究。方法：将TDCS和PDC从肌腱合理和大鼠Achilles筋的植物中分离出来，并培养至第三段。使用双旋转方法从大鼠制备PRP。第三通TDCS和PDC在Dulbecco的改性鹰培养基中培养，用2％胎牛血清（对照组）或2％胎牛血清加5％PRP（PRP组），以及细胞迁移，增殖和分化。通过定量实时检查辛克拉斯（SCX），替氏素（TNMD），胶原型Iα1（COL1A1），胶原蛋白IIIα1（COL3A1），胶原蛋白IIIα1（COL3A1）和血管内皮生长因子A（VEGF）的相对mRNA表达水平逆转录聚合酶链反应。结果：PRP组PDC的细胞迁移率明显高于对照组（增加1.4倍; P = 0.02）。 PRP组的PDC在PDC（增加2.2倍; P <0.01）中显着升高。在PDC中，SCX，COL1A1和VEGF的基因表达水平受PRP（SCX：2.0倍增加，P = 0.01; COL1A1：5.3倍增加，P = 0.01; VEGF：7.8倍增加， P = 0.01），但TNMD的基因表达水平，PRP的肌腱成熟因子显着降低（0.11倍降低; P = 0.02）。结论：在体外PRP促进迁移，增殖和遗传分化与PDC中SCX的上调。 PRP还上调了血管生成标记VEGF的表达。临床相关性：我们的研究结果表明，在体外的PRP治疗可能在肌腱修复初始阶段增强PDC的肌腱愈合特性。

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