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Field application of MALDI-TOF MS on mosquito larvae identification

机译：MALDI-TOF MS对蚊子幼虫鉴定的现场应用

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摘要

In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an efficient tool for arthropod identification. Its application for field monitoring of adult mosquitoes was demonstrated, but identification of larvae has been limited to laboratory-reared specimens. Study aim was to test the success of MALDI-TOF MS in correctly identifying mosquito larvae collected in the field. Collections were performed at 13 breeding sites in urban areas of Marseille, a city in the South of France. A total of 559 larvae were collected. Of these, 73 were accurately morphologically identified, with confirmation either by molecular identification (n = 31) or analysis with MALDI-TOF MS (n = 31) and 11 were tested using both methods. The larvae identified belonged to six species including Culiseta longiareolata, Culex pipiens pipiens, Culex hortensis, Aedes albopictus, Ochlerotatus caspius and Anopheles maculipennis. A high intra-species reproducibility and inter-species specificity of whole larva MS spectra was obtained and was independent of breeding site. More than 92% of the remaining 486 larvae were identified in blind tests against the MS spectra database. Identification rates were lower for early and pupal stages, which is attributed to lower protein abundance and metamorphosis, respectively. The suitability of MALDI-TOF MS for mosquito larvae identification from the field has been confirmed.
机译：近年来，致基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）作为节肢动物鉴定的有效工具。其对成人蚊子的现场监测的应用，但幼虫的鉴定仅限于实验室饲养的标本。研究目的是测试MALDI-TOF MS的成功在正确识别领域收集的蚊虫幼虫。收集在法国南部的一个城市马赛的13个育种地点进行。共收集了总共559个幼虫。其中73重复鉴定，通过分子鉴定（n = 31）或用MALDI-TOF MS（n = 31）和11进行分析，使用两种方法进行确认。幼虫鉴定属于六种物种，包括Culiseta Longiareolata，Culex pipiens pipiens，Culex Hortensis，Aedes Albopictus，Ochlerotatus Caspius和anopheles Maculipenis。获得了高内含物的再现性和整个幼虫MS光谱的特异性，并与育种位点无关。在对抗MS Spectra数据库的盲试验中鉴定了超过92％的剩余486只幼虫。早期和蛹阶段的鉴定率降低，分别归因于蛋白质丰度和变态。已经证实了MALDI-TOF MS对蚊子幼虫的适用性已经证实。

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  • 来源
    《Parasitology》 |2018年第5期|共11页
  • 作者

    AMIRANEBBAK; SEKOUKOUMARE; ALEXANDRA C.WILLCOX; JEAN-MICHELBERENGER; DIDIERRAOULT; LIONELALMERAS; PHILIPPEPAROLA;

  • 作者单位

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

    Aix Marseille Université Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 寄生虫病;
  • 关键词

    Mosquito larvae identification; MALDI-TOF MS;

    机译：蚊子幼虫鉴定;Maldi-Tof MS;

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