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首页> 外文期刊>Plant Cell Reports >IbMPK3/IbMPK6-mediated IbSPF1 phosphorylation promotes tolerance to bacterial pathogen in sweetpotato
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IbMPK3/IbMPK6-mediated IbSPF1 phosphorylation promotes tolerance to bacterial pathogen in sweetpotato

机译：IBMPK3 / IBMPK6介导的IBSPF1磷酸化促进甘薯细菌病原体的耐受性

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摘要

Key message IbSPF1, a novel target of IbMPK3/IbMPK6, regulates biotic stress response in sweetpotato. Environmental stresses due to biotic and abiotic factors negatively affect crop quality and productivity. To minimize the damage caused by these factors, numerous stress signaling pathways are activated in plants. Among these, the mitogen-activated protein kinase (MAPK) signaling cascade plays a pivotal role in diverse plant stress responses. MPK3 and MPK6 function in several cellular signaling pathways by phosphorylating downstream partner proteins in response to environmental stresses. However, little is known about the MPK3/MPK6 signaling pathway in sweetpotato [Ipomoea batatas (L.) Lam]. We recently confirmed that IbMPK3 and IbMPK6, two pathogen-responsive MAPKs, play essential roles in defense gene activation in sweetpotato. In this study, we show that sweetpotato SP8-binding factor (IbSPF1), a substrate of IbMPK3/IbMPK6, functions as a transcriptional regulator of biotic stress signaling in sweetpotato. IbSPF1 specifically interacts with IbMPK3 and IbMPK6, which phosphorylate Ser75 and Ser110 residues of IbSPF1. This increases the affinity of IbSPF1 for the W-box element in target gene promoters. Additionally, the expression of IbSPF1 was up-regulated under various stress conditions and different hormone treatments involved in plant defense responses. Interestingly, the phospho-mimicking mutant of IbSPF1 showed enhanced resistance to Pseudomonas syringae pv. tabaci, and transient expression of mutant IbSPF1 induced the expression of pathogenesis-related genes. These results indicate that the phosphorylation of IbSPF1 by IbMPK3/IbMPK6 plays a critical role in plant immunity by up-regulating the expression of downstream genes.
机译：关键消息IBSPF1是IBMPK3 / IBMPK6的新颖目标，调节甘薯的生物应力反应。由于生物和非生物因素导致的环境压力对作物质量和生产力负面影响。为了使这些因素引起的损害最小，在植物中激活了许多应力信号通路。其中，丝裂原激活的蛋白激酶（MAPK）信号级联在不同植物应激反应中起着枢转作用。通过磷酸化下游合作蛋白响应于环境应力，MPK3和MPK6在几种细胞信号传导途径中起作用。然而，对于甘薯（Ipomoea Batatas（L.）Lam]的MPK3 / MPK6信号通路知之甚少。我们最近证实，IBMPK3和IBMPK6，两种病原体响应的Mapks，在甘薯的防御基因激活中起着基本作用。在这项研究中，我们表明，SweetPOTATO SP8结合因子（IBSPF1），IBMPK3 / IBMPK6的基板，其用作甘薯的生物应力信号传递调节器。 IBSPF1专门与IBMPK3和IBMPK6进行了交互，其磷酸化SER75和IBSPF1的SER110残基。这增加了IBSPF1对靶基因启动子中的W型盒子元件的亲和力。另外，IBSPF1的表达在各种应激条件下调节，以及参与植物防御反应的不同激素治疗。有趣的是，IBSPF1的磷光突突突突突突突突突抗抗假单胞菌皂苷PV。塔巴酸，突变体IBSPF1的瞬时表达诱导致病生成相关基因的表达。这些结果表明，IBMPK3 / IBMPK6的IBSPF1的磷酸化在通过上游基因的表达来促进植物免疫中的关键作用。

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  • 来源
    《Plant Cell Reports》 |2019年第11期|共13页
  • 作者

    Kim Ho Soo; Bian Xiaofeng; Lee Chan-Ju; Kim So-Eun; Park Sung-Chul; Xie Yizhi; Guo Xiaodong; Kwak Sang-Soo;

  • 作者单位

    KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Jiangsu Acad Agr Sci Prov Key Lab Agrobiol Inst Food Crops Nanjing 210014 Jiangsu Peoples R China;

    KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Jiangsu Acad Agr Sci Prov Key Lab Agrobiol Inst Food Crops Nanjing 210014 Jiangsu Peoples R China;

    Jiangsu Acad Agr Sci Prov Key Lab Agrobiol Inst Food Crops Nanjing 210014 Jiangsu Peoples R China;

    KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物细胞学;
  • 关键词

    Sweetpotato; Biotic stress; WRKY transcription factor; IbMPK; IbSPF1;

    机译：甘薯;生物应激;Wrky转录因子;IBMPK;IBSPF1;

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