• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Disease >Detection and Identification of Phytoplasmas in the 16SrIV-A, -B, and -D Subgroups in Palms in Tabasco, Mexico
【24h】

Detection and Identification of Phytoplasmas in the 16SrIV-A, -B, and -D Subgroups in Palms in Tabasco, Mexico

机译：墨西哥塔巴斯科棕榈棕榈树棕榈树植物中植物植物的检测及鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 16SrIV-A phytoplasmas are associated with the devastating disease lethal yellowing (LY) of palms. In Tabasco (Mexico), the death of Cocos nucifera, Adonidia merrillii, and Attalea butyracea palms have been suspected to be associated with LY based on symptomatology. Samples from the trunk of both symptomatic and nonsymptomatic palms were collected in three different environments: two species of palms within a rural zone and the other within an urban zone. DNA was extracted to perform a nested PCR with phytoplasma primers P1/P7-LY16SF/R16R2. A 1,345-bp fragment was amplified from the DNA extracted from each of the 29 LY-symptomatic palms sampled. Phytoplasma identification was achieved by amplicon sequencing and virtual restriction fragment length polymorphism analyses. Three 16SrIV phytoplasma subgroups were detected: 16SrIV-A in C. nucifera, 16SrIV-B in A. merrillii, and 16SrIVD in C. nucifera, A. merrillii, and A. butyracea. Phylogenetic analysis showed also that the three phytoplasma strains found in the palm species clustered with phytoplasmas reported in the literature in the three subgroups identified. This is the first report of phytoplasmas associated with these palm species in Tabasco.
机译：16SRIV-一种植物与令人抗损伤的疾病致命致命（LY）的棕榈树脂。在Tabasco（墨西哥）中，已怀疑基于症状学的LY与LY相关的Cocos Nucifera，Adonidia Merrillii和Attalea Palm的死亡。在三种不同的环境中收集了来自症状和非症状棕榈树干的样本：农村区内的两种棕榈树，另一个城市区内。萃取DNA以用柔软的植物引物进行嵌套的PCR P1 / P7-LY16SF / R16R 2。从29种症状棕榈树中的每一个采集的DNA中扩增1,345bp片段。通过扩增子测序和虚拟限制片段长度多态性分析来实现植物形成鉴定。检测到三个16SRIV植物植物亚组：16SRIV-A In Nucifera，16SRIV-B中的A.Merrillii，16sRIVD在C. Nucifera，A.Merrillii和A.丁特拉西。系统发育分析表明，在鉴定的三个亚组中，棕榈种中发现的棕榈种中发现的三种植物菌株与植物中报道的植物中报道。这是与塔巴斯科的这些棕榈种相关的植物的第一报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号