• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Integrated Plant Diseases Management for Sustainable Agriculture >Phytoplasma diseases of coconut palm in Africa
【24h】

Phytoplasma diseases of coconut palm in Africa

机译：非洲椰子棕榈的植物体疾病

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Coconut lethal diseases such as Cape St. Paul wilt in Ghana, Awka disease in Nigeria and coconut lethal disease in Tanzania, Kenya and Mozambique have killed tens of millions of palms and caused severe economic hardship to poor people who depend directly on the coconut palm for their livelihood. These diseases are caused by phytoplasmas, non-culturable obligate pathogens that colonise the phloem sieve elements of the palm vascular bundles. Using the polymerase chain reaction the 16S rRNA genes and the 16S-23S spacer regions of phytoplasmas associated with these lethal coconut diseases in Africa were amplified. Following sequencing of the rDNA products two primers were designed which were specific for the diseases found in either east or west Africa.Neither of these primers, when paired with a universal primer, produced PCR amplification products from healthy coconut DNA, infected coconut DNA from the Caribbean or DNA from a variety of periwinkle-maintained phytoplasmas. Sequence analysis of the coconut 16S rDNA has shown that the phytoplasmas found in west Africa have greater than 99% similarity, while the similarity between the phytoplasmas of east and west Africa was 953%. Phylogenetic analysis confirmed that these coconut phytoplasmas form a primary cluster within the phytoplasma clade and that the pathogen causing the diseases in west Africa formed a new sub-clade within this cluster.
机译：椰子致死疾病，如坦桑尼亚的尼日利亚的Awka病，肯尼亚和莫桑比克的Awka病，肯尼亚和莫桑比克患者杀死了数百万棕榈树，并对直接依赖于椰子掌的贫困人士造成严重的经济困难他们的生计。这些疾病是由植物，非培养的迫使病原体引起的，该病原体植入棕鼠血管束的韧皮筛子元素。使用聚合酶链反应，16S rRNA基因和与非洲这些致死的椰子疾病相关的植物的16S-23S间隔区域被扩增。在RDNA产物的测序后，设计了两种引物，该引物是针对在东非或西非的疾病的特异性。当与通用底漆配对时，当与普通底漆配对时，从健康的椰子DNA与来自健康椰子DNA的PCR扩增产物，来自来自各种Periwinkle维持的植物的加勒比海或DNA。椰子16S rDNA的序列分析表明，西非发现的植物相似度大于99％，而东部和西非的植物之间的相似性为953％。系统发育分析证实，这些椰子植物在植物植物中形成了植物中的原代簇，使西非疾病的病原体形成了该集群中的新子地区。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号