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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal
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Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal

机译：使用MALDI-TOF MS进行鉴定乍得蚊子和血粉的起源

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摘要

Matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a clinical microbiology tool for the systematic identification of microorganisms. It has recently been presented as an innovative tool for the rapid and accurate identification of mosquitoes and their blood meal. To evaluate the capacity of this tool to identify mosquitoes collected in a tropical environment and preserved with silica gel, we analyzed 188 mosquitoes of different species collected in Chad, which were preserved with silica gel for 2 months. The MALDI-TOF MS analysis correctly identified 96% of the mosquitoes and 37.5% of their blood meals. Using MALDI-TOF MS and molecular biology, eight mosquito species were identified, including Anopheles gambiae s.l., Anopheles rufipes, Culex quinquefasciatus, Culex neavei, Culex pipiens, Culex perexiguus, Culex rima, and Culex watti. Blood meal identification revealed that mosquitoes fed mainly on humans, birds, and cows. Matrix-assisted desorption/ionization time-of-flight mass spectrometry appears to be a promising, fast, and reliable tool to identify mosquitoes and the origin of their blood meal for samples stored with silica gel.
机译：基质辅助解吸/电离飞行时间质谱（MALDI-TOF MS）是用于系统鉴定微生物的临床微生物工具。它最近被呈现为一种创新工具，以便快速准确地识别蚊子及其血液。为了评估该工具识别在热带环境中收集并用硅胶保存的蚊子的能力，我们分析了188名在乍得中收集的不同物种的蚊子，其用硅胶保存2个月。 MALDI-TOF MS分析正确地确定了96％的蚊子和37.5％的血粉。使用MALDI-TOF MS和分子生物学，鉴定了八种蚊虫物种，包括anopheles rufipes，Quopheles rufipes，Culex Quinquefasciatus，Culex Neavei，Culexiguus，Culex Perexiguus，Culex Rima和Culex Watti。血液餐鉴定显示蚊子主要喂养人，鸟类和奶牛。矩阵辅助解吸/电离飞行时间质谱仪似乎是识别蚊子和含有硅胶储存的样品的蚊子和血粉的起源的有希望的快速和可靠的工具。

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