...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Super-resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices
【24h】

Super-resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices

机译：用三维结构照明显微镜在清除小鼠脑切片中使用三维结构照明显微镜的超分辨率结构分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Three-dimensional (3D) super-resolution microscopy technique structured illumination microscopy (SIM) imaging of dendritic spines along the dendrite has not been previously performed in fixed tissues, mainly due to deterioration of the stripe pattern of the excitation laser induced by light scattering and optical aberrations. To address this issue and solve these optical problems, we applied a novel clearing reagent, LUCID, to fixed brains. In SIM imaging, the penetration depth and the spatial resolution were improved in LUCID-treated slices, and 160-nm spatial resolution was obtained in a large portion of the imaging volume on a single apical dendrite. Furthermore, in a morphological analysis of spine heads of layer V pyramidal neurons (L5PNs) in the medial prefrontal cortex (mPFC) of chronic dexamethasone (Dex)-treated mice, SIM imaging revealed an altered distribution of spine forms that could not be detected by high-NA confocal imaging. Thus, super-resolution SIM imaging represents a promising high-throughput method for revealing spine morphologies in single dendrites.
机译：三维（3D）超分辨率显微镜技术结构化照明显微镜（SIM）沿着树突沿着树突状刺的成像尚未在固定组织中进行，主要是由于光散射诱导的激发激光的条纹图案的劣化光学像差。为了解决这个问题并解决这些光学问题，我们将新型清除试剂透光施加到固定的大脑上。在SIM成像中，在荧光处理的切片中改善穿透深度和空间分辨率，并且在单个顶端树突上的大部分成像体积中获得160nm的空间分辨率。此外，在慢性地塞米松（DEX）-Treated小鼠的内侧前甲基葡萄球菌（L5PNS）中的v型金字塔神经元（L5PNS）的脊髓头的形态分析中，SIM成像显示出无法检测到的脊柱形式的改变分布高NA共焦成像。因此，超分辨率SIM成像表示具有揭示单树枝状物中的脊柱形态的有希望的高通量方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号