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首页> 外文期刊>The European Journal of Neuroscience >Segregated labeling of olfactory bulb projection neurons based on their birthdates
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Segregated labeling of olfactory bulb projection neurons based on their birthdates

机译：基于其出生率分离嗅灯泡投影神经元的标记

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Mitral and tufted cells are the projection neurons of the olfactory bulb (OB). We previously reported that somata location and innervation patterns were different between early- and late-born mitral cells (Imamura etal., 2011). Here, we introduced a plasmid that drives the expression of a GFP gene into the mouse OB using in utero electroporation, and demonstrated that we can deliver the plasmid vectors into distinct subsets of OB projection neurons by changing the timing of electroporation after fertilisation. The electroporation performed at embryonic day (E)10 preferentially labeled mitral cells in the accessory OB and main OB mitral cells in dorsomedial mitral cell layer (MCL). In contrast, the E12 electroporation introduced the plasmid vectors preferentially into main OB mitral cells in the ventrolateral MCL and tufted cells. Combining these data with BrdU injections, we confirmed that E10 and E12 electroporation preferentially labeled early- and late-born projection neurons, respectively. This work introduces a novel method for segregated labeling of mouse olfactory bulb projection neurons based on their birthdates. With this technique we found that early- and late-born projection neurons extend their secondary dendrites in the deep and superficial external plexiform layer (EPL), respectively. Although a similar segregation has been suggested for mitral vs. tufted cell dendrites, we found mitral cells projecting secondary dendrites into the superficial EPL in E12-electroporated main OB. Our observations indicate that timing of neurogenesis regulates not only somata location and innervation patterns but also the laminar organisation of projection neuron dendrites in the EPL.
机译：二尖瓣和簇绒细胞是嗅灯泡（OB）的投影神经元。我们之前报道，早期和晚期二尖瓣细胞之间的躯体位置和支配模式不同（Imamura etal。，2011）。在这里，我们介绍了一种质粒，该质粒将GFP基因的表达驱动到小鼠OB中使用子宫电穿孔，并证明我们可以通过改变受精后电穿孔的时序将质粒载体输送到OB投影神经元的不同亚群。在胚胎天（e）10在背侧二尖瓣细胞层（MCL）中的胚胎D天（E）10在辅助性OB和主OB二尖瓣细胞中进行的电穿孔优先标记。相反，E12电穿孔优先将质粒载体引入介口外部MCL和簇绒细胞中的主OB二尖瓣细胞中。将这些数据与BRDU注射结合，我们证实了E10和E12电穿孔分别标记为早期和晚期投影神经元。本作品介绍了一种基于其出生率的小鼠嗅灯泡投影神经元的偏析标记的新方法。利用这种技术，我们发现早期和后期突出的突起神经元分别在深层和表面外部丛状层（EPL）中延伸它们的次级树枝状体。虽然已经提出了类似的偏移对簇绒细胞枝晶，但我们发现将次级枝晶突出的二尖瓣细胞突出到E12电穿孔主OB中的浅层EPL中。我们的观察结果表明神经发生的时序不仅调节了Somata位置和支配模式，而且还调节EPL中投影神经元树枝状的层流组织。

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