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首页> 外文期刊>The European Journal of Neuroscience >Glutamatergic and dopaminergic neurons in the mouse ventral tegmental area
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Glutamatergic and dopaminergic neurons in the mouse ventral tegmental area

机译：小鼠腹侧区域的谷氨酸和多巴胺能神经元

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摘要

The ventral tegmental area (VTA) comprises dopamine (DA), -aminobutyric acid (GABA) and glutamate (Glu) neurons. Some rat VTA Glu neurons, expressing vesicular glutamate transporter 2 (VGluT2), co-express tyrosine hydroxylase (TH). While transgenic mice are now being used in attempts to determine the role of VGluT2/TH neurons in reward and neuronal signaling, such neurons have not been characterized in mouse tissue. By cellular detection of VGluT2 mRNA and TH immunoreactivity (TH-IR), we determined the cellular expression of VGluT2 mRNA within VTA TH-IR neurons in the mouse. We found that some mouse VGluT2 neurons coexpressed TH-IR, but their frequency was lower than in the rat. To determine whether low expression of TH mRNA or TH-IR accounts for this low frequency, we evaluated VTA cellular coexpression of TH transcripts and TH protein. Within the medial aspects of the VTA, some neurons expressed TH mRNA but lacked TH-IR; among them a subset coexpressed VGluT2 mRNA. To determine if lack of VTA TH-IR was due to TH trafficking, we tagged VTA TH neurons by Cre-inducible expression of mCherry in TH::Cre mice. By dual immunofluorescence, we detected axons containing mCherry, but lacking TH-IR, in the lateral habenula, indicating that low frequency of VGluT2 mRNA (+)/TH-IR (+) neurons in the mouse is due to lack of synthesis of TH protein, rather than TH protein trafficking. In conclusion, VGluT2 neurons are present in the rat and mouse VTA, but they differ in the populations of VGluT2/TH and TH neurons. Under normal conditions, the translation of TH protein is suppressed in the mouse mesohabenular TH neurons.
机译：腹侧特蛋白区域（VTA）包含多巴胺（DA）， - 氨基丁酸（GABA）和谷氨酸（Glu）神经元。一些大鼠VTA Glu神经元，表达凹凸谷氨酸转运蛋白2（Vglut2），共表达酪氨酸羟化酶（Th）。虽然现在用于确定VGLut2 / Th神经元在奖励和神经元信号传导中的作用的转基因小鼠，但这种神经元尚未在小鼠组织中表征。通过细胞检测Vglut2 mRNA和Th Th免疫反应性（TH-IR），我们确定小鼠VTA TH-IR神经元中VGLut2 mRNA的细胞表达。我们发现一些小鼠Vglut2神经元共同表达TH-IR，但它们的频率低于大鼠。为了确定TH mRNA或TH-IR的低表达是否占这种低频的估计，我们评估了TH转录物和TH蛋白的VTA细胞共表达。在VTA的内侧方面，一些神经元表达了TH mRNA但缺乏TH-IR;其中包括子集共用VGLut2 mRNA。为了确定缺乏VTA TH-IR是否是由于贩运，我们在TH :: CRE小鼠中通过CRE-Imcucible的敏锐表达标记了VTA Th神经元。通过双免疫荧光，我们检测到含有MCHERRY的轴突，但缺乏TH-IR，表明鼠标中VGLUT2 mRNA（+）/ TH-IR（+）神经元的低频率是由于缺乏合成蛋白质，而不是蛋白质贩运。总之，Vglut2神经元存在于大鼠和小鼠VTA中，但它们在Vglut2 / Th和Th神经元的群体中不同。在正常条件下，在小鼠中象牙子宫中抑制了Th蛋白的翻译。

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