...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Storage of orchid pollinia with varying lipid thermal fingerprints
【24h】

Storage of orchid pollinia with varying lipid thermal fingerprints

机译：用不同的脂质热指纹储存兰花小花花体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Orchid pollinia have the potential to make a valuable contribution to current techniques of germplasm storage and assisted reproduction, yet information regarding their preservation and their ability to remain viable over time is currently limited. Dactylorhiza fuchsii and Disa uniflora were used as models for investigating potential techniques for storing orchid pollinia. Initially, freshly harvested pollinia of Dact. fuchsii were incubated at 25 degrees C and 100% RH (relative humidity) for up to 7 days and germinated in vitro. For pollinia from both species, moisture sorption isotherms were constructed and thermal fingerprints generated using differential scanning calorimetry (DSC). Pollinia were stored at three temperatures (5, - 18 and - 196 degrees C) after equilibration at four different RHs (5, 33, 50 and 75%) and germinated. The isotherms and DSC results varied between species. Compared with D. uniflora, pollinia of Dact. fuchsii consistently equilibrated at higher moisture content (MC) for each RH, had less detectable lipids by DSC and had shorter lifespans, remaining viable after 3-4 months only at - 20 and - 196 degrees C and at low RH (5 and 33%). Both species' pollinia stored well at - 20 degrees C and - 196 degrees C, although there was some evidence of a small loss of viability under cryopreservation. In conclusion, pollen of these two species can be stored successfully for at least 3-4 months, and to maximize the pre-storage quality, it is recommended that fresh pollen is collected from flowers just prior to anthesis.
机译：兰花小组有可能对目前的种质储存和协助繁殖技术进行有价值的贡献，但有关其保存的信息及其在随着时间的推移保持可行的能力和能力的信息目前有限。 DACTYLORHIZA FUCHSII和DISA UNOVLORA被用作调查用于储存兰花POLLINIA的潜在技术的模型。最初，新鲜收获的Dact Poldinia。将Fuchsii以25℃和100％RH（相对湿度）孵育长达7天，并在体外发芽。对于从种物种的Pollinia，构造了水分吸附等温线，并使用差示扫描量热法（DSC）产生的热指纹。在四种不同RHS（5,33,50和75％）的平衡后，在三个温度（5， - 18和-196摄氏度）下储存了花粉素并发芽。等温线和DSC结果在物种之间变化。与D. Uniflora相比，Dact的Pollinia。对于每rH的较高水分含量（MC）持续平衡，DSC具有较小的可检测的脂质并具有较短的寿命，并且在-20和-196摄氏度和低温下仅3-4个月后仍然可行（5和33％） ）。虽然在冷冻保存下存在一些证据表明，但在20摄氏度和-196摄氏度下储存了井 - 20摄氏度的储存良好。总之，这两种物种的花粉可以成功储存至少3-4个月，并最大限度地提高预储存质量，建议在开花前从花卉收集新鲜的花粉。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号