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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Isolation and structural elucidation of pelgipeptin E, a novel pore-forming pelgipeptin analog from Paenibacillus elgii with low hemolytic activity
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Isolation and structural elucidation of pelgipeptin E, a novel pore-forming pelgipeptin analog from Paenibacillus elgii with low hemolytic activity

机译：Pelgipeptin E的分离和结构阐明，一种具有低溶血活性的Paenibacillus Elgii的新型孔隙形成骨盆类似物

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摘要

Pelgipeptins are cyclic lipopeptides composed of nine amino acids and a short fatty acid chain. In the present study, we report a novel pelgipeptin peptide that was isolated from Paenibacillus elgii BC34-6 and named pelgipeptin E (PGP-E). The molecular mass of PGP-E was 1072 Da as determined by liquid chromatography-mass spectrometry and the amino acid sequence was elucidated by tandem mass spectrometry. The complete molecular structure of PGP-E was characterized using 2D NMR spectroscopy. PGP-E consisted of a cyclic peptide backbone of Dab1-Va12-Dab3-Phe4-Leu5-Dab6-Va17-Leu8-Ser9 and a lipid chain (-CH2CH2CH3). PGP-E had broad antimicrobial activity against gram-negative and -positive bacteria, including methicillin-resistant Staphylococcus aureus strains. Furthermore, the mode of action of PGP-E was investigated using calcein dye leakage and membrane depolarization assays, which suggest that PGP-E acts via a membrane-active mechanism. The hemolytic activity of PGP-E was significantly lower than that of melittin, a well-known membrane-active peptide derived from bee venom. These results suggest that PGP-E is a potential candidate in the development of new peptide antibiotics.
机译：Pelgipeptins是由九个氨基酸和短脂肪酸链组成的环状脂肽。在本研究中，我们报告了一种从Paenibacillus Elgii BC34-6中分离的新型Pelgipeptin肽，并命名为pelgipeptine（pgp-e）。通过液相色谱 - 质谱法测定PGP-E的分子量为1072Da，通过串联质谱法阐明氨基酸序列。使用2D NMR光谱表征PGP-E的完整分子结构。 PGP-E由DAB1-VA12-DAB3-PHE4-LEU5-DAB6-VA17-LEU8-SER9和脂质链（-CH2CH2CH3）的环状肽骨架组成。 PGP-E对革兰蛋白抗性细菌具有广泛的抗微生物活性，包括耐甲氧西林金黄色葡萄球菌株。此外，使用Calcein染料泄漏和膜去极化测定来研究PGP-E的作用方式，这表明PGP-E通过膜 - 活性机制起作用。 PGP-E的溶血活性显着低于甜瓜，衍生自蜂毒液的众所周知的膜活性肽。这些结果表明，PGP-E是新肽抗生素的发展中的潜在候选者。

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    Kim Jueun; Kim Pyoung Il; Bong Ki Moon; Kim Jae Il; Shin Song Yub; Song Jaekyeong; Min Hye Jung; Lee Chul Won;

  • 作者单位

    Chonnam Natl Univ Dept Chem Gwangju 61186 South Korea;

    Ctr Industrializat Agr &

    Livestock Microorganisms Jeongyup 56212 Chonbuk South Korea;

    Natl Inst Environm Res Youngsan River Environm Res Ctr Gwangju 61011 South Korea;

    Gwangju Inst Sci &

    Technol Dept Life Sci Gwangju 61005 South Korea;

    Chosun Univ Dept Med Sci Grad Sch Gwangju 61452 South Korea;

    Rural Dev Adm Natl Inst Agr Sci NAS Agr Microbiol Div Jeonju 55365 South Korea;

    Kwangju Womens Univ Dept Cosmet Sci Gwangju 62396 South Korea;

    Chonnam Natl Univ Dept Chem Gwangju 61186 South Korea;

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  • 正文语种 eng
  • 中图分类 药学;
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