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首页> 外文期刊>The Journal of Antibiotics: An International Journal >In vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus.
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In vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus.

机译：克拉胺的体外抗菌和溶血活性，来自响尾蛇克罗塔卢萨斯的小碱性肌毒素。

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摘要

Crotamine, a myotoxin from the venom of South American rattlesnake, is structurally related to beta-defensins, antimicrobial peptides (AMPs) found in vertebrate animals. Here, we tested the antibacterial properties of crotamine and found that it killed several strains of Escherichia coli, with the MICs ranging from 25 to 100 mug ml(1). Time-kill and bacterial membrane permeabilization assays revealed that killing of bacteria by crotamine occurred within 1 h and reached the maximum by 2 h. Additionally, the anti-E. coli activity of crotamine was completely abolished with 12.5 mM NaCl. Furthermore, the three intramolecular disulfide bonds of crotamine appeared dispensable for its antibacterial activity. The reduced form of crotamine was active against E. coli as well. However, crotamine showed no or weak activity up to 200 mug ml(1) against other species of Gram-negative and Gram-positive bacteria. Crotamine showed no appreciable hemolytic activity to erythrocytes. Our studies revealed that crotamine is also an AMP that kills bacteria through membrane permeabilization. However, crotamine appears to have a narrow antibacterial spectrum, distinct from many classical beta-defensins, reinforcing the notion that crotamine originated from the beta-defensin gene lineage, but has undergone significant functional diversification.
机译：来自南美洲响尾蛇毒液的肌毒素的肌毒素在结构上与β-防御素（AMP）在脊椎动物中发现。在这里，我们测试了克罗丁胺的抗菌性质，发现它杀死了几种大肠杆菌菌株，其中麦克风范围为25至100麦克风（1）。杀死和细菌膜透露测定显示，克拉胺杀死细菌在1小时内发生，并达到最大2小时。另外，反e。克拉胺的大肠杆菌活性完全废除12.5mm NaCl。此外，对其抗菌活性的三个分子内二硫键出现了可分配的。还减少了克罗酪甲酰胺对大肠杆菌的活性。然而，克罗辛胺显示出没有或弱的活性，可对抗其他革兰氏阴性和革兰氏阳性细菌的200麦克风（1）。克拉胺向红细胞显示出明显的溶血活性。我们的研究表明，克罗辛也是通过膜透透化杀死细菌的放大器。然而，克罗那似乎具有狭窄的抗菌谱，不同于许多经典β-防御素，加强了克拉酪蛋白源于β-防御素基因谱系的观念，而是经过显着的功能多样化。

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