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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Characterization of the biosynthetic gene cluster (ata) for the A201A arninonucleoside antibiotic from Saccharothrix mutabilis subsp capreolus
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Characterization of the biosynthetic gene cluster (ata) for the A201A arninonucleoside antibiotic from Saccharothrix mutabilis subsp capreolus

机译：来自Saccharothrix Mutabilis患者A201A芳族核苷抗生素的生物合成基因簇（ATA）的表征

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摘要

Antibiotic A201A produced by Saccharothrix mutabilis subsp. capreolus NRRL3817 contains an aminonucleoside (N-6, N-6-dimethy1-3'-amino-3'-deoxyadenosyl), a polyketide (a-methyl-p-coumaric acid) and a disaccharide moiety. The heterologous expression in Streptomyces lividans and Streptomyces coelicolor of a S. mutabilis genomic region of similar to 34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (ata). The analysis of the nucleotide sequence of the fragment reveals the presence of 32 putative open reading frames (ORF), 28 of which according to boundary gene inactivation experiments are likely to be sufficient for A201A biosynthesis. Most of these ORFs could be assigned to the biosynthesis of the antibiotic three structural moieties. Indeed, five ORFs had been previously implicated in the biosynthesis of the aminonucleoside moiety, at least nine were related to the biosynthesis of the polyketide (ata-PKS1-ataPKS4, ata18, ata19, ata2, ata4 and ata7) and six were associated with the synthesis of the disaccharide (ata12, ata13, ata16, ata17, ata5 and ata 10) moieties. In addition to AtaP5, three putative methyltransferase genes are also found in the ata cluster (Ata6, Ata8 and Ata 11), and no regulatory genes were found.
机译：由Saccharothrix Mutabilis亚数据生产的抗生素A201a。 Capreolus NRR13817含有氨基核苷（N-6，N-6-二甲基二甲基1-3'-氨基-3'-脱氧糖基糖基），聚酮（A-甲基-P-香豆酸）和二糖部分。 S. mutabilis基因组区域的链霉菌和链霉菌的异源表达类似于34kb的Mutabilis基因组区域，导致通过微生物，生物化学和物理化学方法鉴定的A201a，并表明该区域可能含有整个A201A生物合成基因簇（ATA）。片段的核苷酸序列的分析显示，根据边界基因失活试验的32个推定的开放阅读框架（ORF），28的存在可能足以用于A201A生物合成。这些ORF中的大多数可以分配给抗生素三个结构部分的生物合成。实际上，预先在氨基核苷部分的生物合成中涉及五个ORF，至少九个与聚酮化酮的生物合成有关（ATA-PKS1-ATAPKS4，ATA18，ATA19，ATA2，ATA4和ATA7），六个与之相关二糖的合成（ATA12，ATA13，ATA16，ATA17，ATA5和ATA 10）部分。除了ATAP5之外，还在ATA簇（ATA6，ATA8和ATA 11）中发现了三个推定的甲基转移酶基因，并且没有发现该调节基因。

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    Saugar Irene; Molloy Brian; Sanz Eloisa; Sanchez Maria Blanca; Fernandez-Lobato Maria; Jimenez Antonio;

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    Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM Dept Biol Mol Nicolas Cabrera 1 E-28049;

    Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM Dept Biol Mol Nicolas Cabrera 1 E-28049;

    Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM Dept Biol Mol Nicolas Cabrera 1 E-28049;

    Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM Dept Biol Mol Nicolas Cabrera 1 E-28049;

    Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM Dept Biol Mol Nicolas Cabrera 1 E-28049;

    Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM Dept Biol Mol Nicolas Cabrera 1 E-28049;

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  • 正文语种 eng
  • 中图分类 药学;
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