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首页> 外文期刊>The Journal of Antibiotics: An International Journal >A choline binding polypeptide of LytA inhibits the growth of Streptococcus pneumoniae by binding to choline in the cell wall
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A choline binding polypeptide of LytA inhibits the growth of Streptococcus pneumoniae by binding to choline in the cell wall

机译：Lyta的胆碱结合多肽通过在细胞壁中与胆碱结合而抑制链球菌肺炎酸的生长

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摘要

Streptococcus pneumoniae is a pathogen that mainly affects children and elderly individuals. The numerous serotypes and increased resistance to antibiotics make the treatment of pneumococcal infections sometimes difficult. Asymptomatic colonization is the main reservoir for S. pneumoniae, but no vaccine or antibiotic treatment is effective in eliminating this reservoir. Here, we show that a simulated choline binding polypeptide (ChBp) of LytA has antimicrobial activity against S. pneumoniae. ChBp showed specific antimicrobial activity against pneumococcal but not against non-streptococcal strains, and no cytotoxic effect was observed for 293t cell. The minimal inhibitory concentration (MIC) is between 10-25 mu g/ml. In addition, we found ChBp functions by binding to the choline in the cell wall with a binding capacity between 3.25 and 7.5 x 10(-6)g/CFU. The binding cannot kill, but can inhibit the growth of pneumococcal cells for up to 12 h (50 mu g/ml). Viable cells were decreased by 50% at 18 h, and eliminated at 36 h of incubation. These results show that ChBp has potential for the treatment of pneumococcal disease, or for eliminating nasopharyngeal colonization.
机译：肺炎链球菌是一种病原体，主要影响儿童和老年人。许多血清型和对抗生素的抗性增加会使肺炎球菌感染有时难以治疗。无症状殖民化是S.肺炎的主要储层，但没有疫苗或抗生素治疗是有效消除该储层的有效性。在这里，我们表明，Lyta的模拟胆碱结合多肽（Chbp）对S.肺炎具有抗微生物活性。 CHBP显示针对肺炎球菌的特异性抗微生物活性，但不针对非链球菌菌株，并且没有观察到293T细胞的细胞毒性效果。最小抑制浓度（MIC）在10-25μg/ ml之间。此外，我们通过将Chbp与细胞壁中的胆碱结合，在3.25和7.5×10（-6）g / cfu之间的结合能力与细胞壁中的胆碱结合。结合不能杀死，但可以抑制肺炎球菌细胞的生长至多12小时（50μmg/ ml）。在18小时下可行细胞减少50％，并在36小时内孵育。这些结果表明，CHBP具有治疗肺炎球菌病的潜力，或消除鼻咽殖民化。

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    Zhang Zhikun; Zhang Xinzhuo; Zhang Luhua; Wang Guangxi; Zeng Jing; Zhang Jinping; Hu Xiaoyan; Zhou Yingshun;

  • 作者单位

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Int Educ Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

    Southwest Med Univ Sch Basic Med Dept Pathogen Biol Luzhou Sichuan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药学;
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