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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Discovery of 'heat shock metabolites' produced by thermotolerant actinomycetes in high-temperature culture
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Discovery of 'heat shock metabolites' produced by thermotolerant actinomycetes in high-temperature culture

机译：在高温培养中发现热电子放线菌产生的“热休克代谢物”

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In actinomycetes, many secondary metabolite biosynthetic genes are not expressed under typical laboratory culture conditions and various efforts have been made to activate these dormant genes. In this study, we focused on high-temperature culture. First, we examined the thermotolerance of 3160 actinomycete strains from our laboratory culture collection and selected 57 thermotolerant actinomycetes that grew well at 45 degrees C. These 57 thermotolerant actinomycetes were cultured for 5 days in liquid medium at both 30 degrees C and 45 degrees C. Culture broths were extracted with 1-butanol, and each extract was subjected to LC/MS analysis. The metabolic profiles of each strain were compared between the 30 degrees C and 45 degrees C cultures. We found that almost half of these thermotolerant actinomycetes produced secondary metabolites that were detected only in the 45 degrees C culture. This result suggests that high-temperature culture induces the production of dormant secondary metabolites. These compounds were named "heat shock metabolites (HSMs)." To examine HSM production in more detail, 18 strains were selected at random from the initial 57 strains and cultivated in six different media at 30 degrees C and 45 degrees C; as before, metabolic profiles of each strain in each medium were compared between the 30 degrees C and 45 degrees C cultures. From this analysis, we found a total of 131 HSMs. We identified several angucycline-related compounds as HSMs from two thermotolerant Streptomyces species. Furthermore, we discovered a new compound, murecholamide, as an HSM from thermotolerant Streptomyces sp. AY2. We propose that high-temperature culture of actinomycetes is a convenient method for activating dormant secondary metabolite biosynthetic genes.
机译：在放射素瘤中，在典型的实验室培养条件下没有表达许多次级代谢物生物合成基因，并且已经进行了各种努力来激活这些休眠基因。在这项研究中，我们专注于高温培养。首先，从我们的实验室培养收集中检查了3160株放射素菌株的热能，并选择了57个热电子放线菌在45摄氏度下良好。在30℃和45℃的液体培养基中培养5天5天。用1-丁醇萃取培养液，每次提取物进行LC / MS分析。在30摄氏度和45摄氏度培养物之间比较每个菌株的代谢谱。我们发现几乎一半的热电子放线菌产生了仅在45摄氏度中检测到的次生代谢物。该结果表明，高温培养诱导休眠次级代谢物的产生。这些化合物被命名为“热休克代谢物（HSMS）”。为了更详细地检查HSM生产，从初始57株中随机选择18个菌株，并在30℃和45℃下以六种不同的培养基培养;如前所述，在30摄氏度和45摄氏度之间进行比较每种培养基中的每种菌株的代谢谱。从这个分析中，我们发现总共131个HSM。我们将几种与两种热电子链霉菌物种的HSM鉴定了几种与HSMS相关的化合物。此外，我们发现了一种新化合物，Murecholamide，作为来自热调虫链霉菌SP的HSM。 AY2。我们提出了放线菌的高温培养是激活休眠次级代谢物生物合成基因的方便方法。

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    Saito Shun; Kato Wataru; Ikeda Hiroaki; Katsuyama Yohei; Ohnishi Yasuo; Imoto Masaya;

  • 作者单位

    Keio Univ Fac Sci &

    Technol Dept Biosci &

    Informat Yokohama Kanagawa 2238522 Japan;

    Keio Univ Fac Sci &

    Technol Dept Biosci &

    Informat Yokohama Kanagawa 2238522 Japan;

    Keio Univ Fac Sci &

    Technol Dept Biosci &

    Informat Yokohama Kanagawa 2238522 Japan;

    Univ Tokyo Grad Sch Agr &

    Life Sci Dept Biotechnol Bunkyo Ku 1-1-1 Yayoi Tokyo 1138657 Japan;

    Univ Tokyo Grad Sch Agr &

    Life Sci Dept Biotechnol Bunkyo Ku 1-1-1 Yayoi Tokyo 1138657 Japan;

    Keio Univ Fac Sci &

    Technol Dept Biosci &

    Informat Yokohama Kanagawa 2238522 Japan;

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  • 正文语种 eng
  • 中图分类 药学;
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