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首页> 外文期刊>The journal of maternal-fetal & neonatal medicine >Plasma and amniotic fluid PPARy is involved in the lipid metabolism of maternal-fetal interface cells
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Plasma and amniotic fluid PPARy is involved in the lipid metabolism of maternal-fetal interface cells

机译:血浆和羊水PPARY参与母体胎儿界面细胞的脂质代谢

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Objectives: The aim of this study was at detecting the expression of peroxisome proliferator-activated receptor y (PPARgamma) in patients with gestational diabetes mellitus (GDM) and to investigate whether PPARgamma regulates the lipid metabolism of maternal-fetal interface cells. Methods: We detected the expression of PPARgamma in the maternal plasma, fetal plasma and amniotic fluid of two groups of women with GDM or normal pregnancy using ELISA and western blot. Establish the cell models of the indirect coculture model and the separate culture model using BeWo cells and endometrial stromal cells (ESCs). Oil red O staining was used to detect the lipid uptake, and the mRNA levels of the rate-limiting enzyme stearoyl CoA desaturase (SCD) and glucose transporter 1 (GLUT1) were detected by RT-PCR.
机译:目的:本研究的目的是检测妊娠期糖尿病(GDM)患者过氧化物激素激活受体Y(PPARγ)的表达,并研究PPARGAMMA是否调节母体界面细胞的脂质代谢。 方法:使用ELISA和Western印迹检测到两组女性的母体血浆,胎儿血浆和羊水中Pparγ,胎儿血浆和羊水的表达。 使用Bewo细胞和子宫内膜基质细胞建立间接共培养模型的细胞模型和单独的培养模型(ESC)。 用RT-PCR检测油红色O染色来检测脂质摄取,率限制酶硬脂酰CoA去饱和酶(SCD)和葡萄糖转运蛋白1(GLUT1)的mRNA水平。

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