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首页> 外文期刊>The ISME journal emultidisciplinary journal of microbial ecology >Atmospheric carbon monoxide oxidation is a widespread mechanism supporting microbial survival
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Atmospheric carbon monoxide oxidation is a widespread mechanism supporting microbial survival

机译：大气一氧化碳氧化是一种支持微生物存活的广泛机制

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Carbon monoxide (CO) is a ubiquitous atmospheric trace gas produced by natural and anthropogenic sources. Some aerobic bacteria can oxidize atmospheric CO and, collectively, they account for the net loss of similar to 250 teragrams of CO from the atmosphere each year. However, the physiological role, genetic basis, and ecological distribution of this process remain incompletely resolved. In this work, we addressed these knowledge gaps through culture-based and culture-independent work. We confirmed through shotgun proteomic and transcriptional analysis that the genetically tractable aerobic soil actinobacterium Mycobacterium smegmatis upregulates expression of a form I molydenum-copper carbon monoxide dehydrogenase by 50-fold when exhausted for organic carbon substrates. Whole-cell biochemical assays in wild-type and mutant backgrounds confirmed that this organism aerobically respires CO, including at sub-atmospheric concentrations, using the enzyme. Contrary to current paradigms on CO oxidation, the enzyme did not support chemolithoautotrophic growth and was dispensable for CO detoxification. However, it significantly enhanced long-term survival, suggesting that atmospheric CO serves a supplemental energy source during organic carbon starvation. Phylogenetic analysis indicated that atmospheric CO oxidation is widespread and an ancestral trait of CO dehydrogenases. Homologous enzymes are encoded by 685 sequenced species of bacteria and archaea, including from seven dominant soil phyla, and we confirmed genes encoding this enzyme are abundant and expressed in terrestrial and marine environments. On this basis, we propose a new survival-centric model for the evolution of aerobic CO oxidation and conclude that, like atmospheric H-2, atmospheric CO is a major energy source supporting persistence of aerobic heterotrophic bacteria in deprived or changeable environments.
机译：一氧化碳（CO）是由天然和人为源产生的普遍存在的大气痕量气体。一些有氧细菌可以氧化大气的CO和统称，他们每年从大气中的净损失达到相似的净损失。然而，这种过程的生理作用，遗传基础和生态分布仍未完全解决。在这项工作中，我们通过以文化为基础和独立的工作解决了这些知识差距。我们通过霰弹枪蛋白质组学和转录分析证实，转基因的有氧土壤肌动术分枝杆菌的分枝杆菌在耗尽时将形式I钼 - 铜一氧化碳脱氢酶的表达提高50倍，用于有机碳基材。野生型和突变体背景中的全细胞生物化学测定证实，这种有机体有氧地呼吸有限公司，包括使用酶的亚大气浓度。与CO氧化的目前范式相反，酶不支持趋化性营养性生长，并可用于共同解毒。然而，它显着增强了长期存活，表明大气CO在有机碳饥饿期间为辅助能源提供服务。系统发育分析表明，大气共同氧化是广泛的和CO脱氢酶的祖先性状。同源酶由685个测序的细菌和古氏菌编码，包括七种显性土壤培养物，我们确认编码该酶的基因丰富，在陆地和海洋环境中表达。在此基础上，我们提出了一种新的生存以需求为中心为中心的有氧CO氧化的演变和结论，如大气H-2，大气CO是一种剥夺或可变环境中有氧异养细菌的主要能源。

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