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首页> 外文期刊>Biotechnology Progress >Modeling of overflow metabolism in batch and fed-batch cultures ofEscherichia coli
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Modeling of overflow metabolism in batch and fed-batch cultures ofEscherichia coli

机译：大肠杆菌分批和补料分批培养中溢流代谢的建模

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摘要

A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only when specific glucose uptake exceeded a critical rate corresponding to a maximum respiration rate. In batch cultures where the glucose uptake was unlimited, the overflow acetate made up to 9.0 +/- 1.0% carbon/carbon of the glucose consumed. The applicability of the model to dynamic situations was tested by challenging the model with glucose and acetate pulses added during the fed-batch part of the cultures. In the presence of a glucose feed, E. coli utilized acetate 3 times faster than in the absence of glucose. The cells showed no significant difference in maximum specific uptake rate of endogenous acetate produced by glucose overflow and exogenous acetate added to the culture, the value being 0.12-0.18 g g(-1) h(-1) during the entire fed-batch culture period. Acetate inhibited the specific growth rate according to a noncompetitive model, with the inhibition constant (k(i)) being 9 g of acetate/L. This was due to the reduced rate of glucose uptake rather than the reduced yield of biomass.
机译：提出了在完全有氧条件下分批和补料分批培养大肠杆菌W3110的葡萄糖溢流代谢的动力学模型。基于该模型的仿真描述了分批培养，分批向补料分批培养的过渡以及补料分批培养期间细胞的生长，呼吸作用和乙酸盐的形成以及乙酸盐的再消耗。仅当特定的葡萄糖摄取超过对应于最大呼吸速率的临界速率时，大肠杆菌才会排出乙酸盐。在葡萄糖摄取不受限制的分批培养中，溢流乙酸盐占所消耗葡萄糖的9.0 +/- 1.0％碳/碳。通过在培养物的分批补料过程中添加葡萄糖和醋酸盐脉冲挑战模型，测试了模型对动态情况的适用性。在存在葡萄糖进料的情况下，大肠杆菌利用乙酸盐的速度是不存在葡萄糖时的3倍。细胞在葡萄糖上溢和向培养液中添加外源乙酸盐产生的内源乙酸盐的最大比吸收率无显着差异，在整个分批补料培养期间的值为0.12-0.18 gg（-1）h（-1） 。根据非竞争性模型，乙酸盐抑制了特定的生长速率，其抑制常数（k（i））为9 g醋酸盐/ L。这是由于降低了葡萄糖摄取速率，而不是降低了生物质产量。

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