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首页> 外文期刊>Biotechnology Progress >In situ detoxification of the fermentation medium during gamma-decalactoneproduction with the yeast Sporidiobolus salmonicolor
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In situ detoxification of the fermentation medium during gamma-decalactoneproduction with the yeast Sporidiobolus salmonicolor

机译：γ-十内酯生产过程中发酵培养基的原位解毒

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摘要

gamma-Decalactone (gamma-C-10) is known to be highly toxic for the microorganims used for its production. In this work, three techniques were studied in order to overcome this toxicity during a bioconversion process using ricinoleic acid as precursor of the lactone: in situ trapping in oily phases, in porous hydrophobic sorbents and in beta-cyclodextrins. Oily phases added to the media (olive, Miglyol, tributyrin, and paraffin) had a protective effect on Sp. salmonicolor, and they improved the lactone production. beta-cyclodextrins, which have a hydrophobic cavity that can trap molecules such as gamma-C-10, have been used to protect the yeasts. The results showed insufficient preservation of cell viability. Some sorbents (activated carbon and polystyrene-based sorbents) were successfully tested during bioconversion. In all cases viability exceeded the reference one. Nevertheless the aroma production was 30% lower than the reference. All of these solutions led to some enhancement of the cell viability during bioconversion of methyl ricinoleate to gamma-C-10. For improvement of the lactone production, the oil trapping method seemed to be the best with the experimental conditions tested.
机译：已知γ-十内酯（γ-C-10）对用于其生产的微生物有剧毒。在这项工作中，研究了三种技术以克服使用蓖麻油酸作为内酯前体的生物转化过程中的这种毒性：在油相，多孔疏水吸附剂和β-环糊精中原位捕获。添加到培养基中的油相（橄榄，米格洛尔，三丁酸甘油酯和石蜡）对Sp有保护作用。鲑鱼色，它们提高了内酯的产量。 β-环糊精具有疏水腔，可以捕获诸如γ-C-10等分子，已被用于保护酵母。结果显示细胞存活力的保留不足。在生物转化过程中成功地测试了一些吸附剂（活性炭和聚苯乙烯基吸附剂）。在所有情况下，生存能力均超过参考值。但是，香气产生量比参考值低30％。所有这些解决方案都导致蓖麻油酸甲酯生物转化为γ-C-10期间细胞活力有所提高。为了改善内酯的产生，在测试的实验条件下，捕油法似乎是最好的。

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