• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Capture of rare cells in suspension with antibody-coated polystyrene beads
【24h】

Capture of rare cells in suspension with antibody-coated polystyrene beads

机译：用抗体包被的聚苯乙烯珠捕获悬浮液中的稀有细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A method for the separation of one cell type present in small number from a predominant mixture of cell types using macroscopic polystyrene beads is demonstrated. An antibody specific to murine leukocytes (CD45) was adsorbed to the surface of the beads. Beads and murine hybridoma B cells were placed in test tubes and periodically inverted-at fixed time intervals, causing the beads to settle through the suspension under creeping flow conditions. Capture was dependent upon interception: the captured cells must have traveled along streamlines that brought them to within a cell radius of the bead surface. B cells attached to 99-mu m beads (maximum shear rate 8.1 s(-1)) were captured with greater efficiency but in lesser quantity than those attached to 170-mu m beads (maximum shear rate 13.9 s(-1)). Cell capture unexpectedly reached a plateau in less than 2 h, a phenomenon that appears to involve changes in both the cells and the beads. Capture of cells was effective out to dilutions of 1:10 000 with purity in the captured population of better than 74%. This method allows for the study of physical parameters important for cell attachment and capture as well as for practical separation of rare cells.
机译：证明了使用宏观聚苯乙烯珠从主要细胞类型混合物中分离少量存在的一种细胞类型的方法。鼠白细胞（CD45）特异的抗体被吸附到珠子的表面。将珠子和鼠类杂交瘤B细胞放入试管中，并以固定的时间间隔定期倒置，使珠子在蠕动流动条件下通过悬浮液沉降。捕获取决于拦截：捕获的细胞必须沿着流线传播，使它们进入珠子表面的细胞半径内。附着在99微米磁珠上的B细胞（最大剪切速率8.1 s（-1））比附着在170微米磁珠上的B细胞（最大剪切速率13.9 s（-1））效率更高，但数量较少。细胞捕获在不到2小时内出人意料地达到了平稳状态，这种现象似乎涉及细胞和珠粒的变化。捕获的细胞有效稀释至1:10 000的稀释度，捕获的群体中的纯度超过74％。这种方法可以研究对细胞附着和捕获以及对稀有细胞的实际分离很重要的物理参数。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号