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首页> 外文期刊>AIDS Research and Human Retroviruses >HIV type 1 infection among Ethiopian immigrants to Israel: enhanced in vitro antibody stimulation for estimating the length of the window period.
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HIV type 1 infection among Ethiopian immigrants to Israel: enhanced in vitro antibody stimulation for estimating the length of the window period.

机译：以色列埃塞俄比亚移民中的HIV 1型感染：增强体外抗体刺激以估计窗口期的长度。

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The window period between infection and seroconversion varies based on viral genetics, dose and route of transmission, host genetics, and environmental factors. The in vitro Stimmunology blood pretreatment assay was utilized to promote the synthesis of HIV-specific antibodies in efforts to define the window period between infection and seroconversion. Ethiopians seeking immigration to Israel while in refugee camps provided a unique cohort to perform a comparative analysis of the window period between HIV-1 infection and diagnosis utilizing conventional Ab-ELISA and our laboratory established an in vitro Stimmunolog assay. This population was considered unique due to its exposure to an environment with a high seroprevalence rate for a defined period of time. Unlinked blood samples were tested and validated before and after Stimmunology. Diagnostic screening was conducted in Israel. A total of 285 and 537 random samples were tested from the 1992 and 1998 immigrant population, respectively. Analysis of HIV prevalence, incidence, and window period length among the immigrants was measured by comparing results obtained on samples prior to and following analysis by Stimmunology as compared with standard ELISA-based assay. This novel assay that promotes the in vitro stimulation of antibody synthesis led to the diagnosis of 2.7% and 0.36% of the 1992 and 1998 immigrant groups, respectively, as HIV infected individuals during the window period. Using mathematical modeling, the length of the window period in the surveyed population was estimated to range from 2 to 5 months. Stimmunology-aided detection of early seronegative HIV-infected individuals provided a reliable and consistent mode of identifying infection in seronegative HIV-1-infected individuals. Applying mathematical modeling to the data obtained enabled us to define the window period length, which was found to extend beyond previous estimates. These results suggest a need for the reevaluation of the techniques that are employed to assess the true incidence and prevalence of HIV-1 infection, especially in populations within sub-Saharan Africa.
机译：感染和血清转化之间的窗口期根据病毒遗传学，传播的剂量和途径，宿主遗传学和环境因素而异。为了确定感染和血清转化之间的窗口期，采用了体外Stimmunology血液预处理测定法来促进HIV特异性抗体的合成。埃塞俄比亚人在难民营中寻求移民到以色列提供了一个独特的队列，可以利用传统的Ab-ELISA对HIV-1感染和诊断之间的窗口期进行比较分析，我们的实验室建立了体外Stimmunolog分析。该种群被认为是独特的，因为它在规定的时间内暴露于高血清阳性率的环境中。在Stimmunology之前和之后对未链接的血液样本进行测试和验证。在以色列进行了诊断筛查。从1992年和1998年的移民人口中分别测试了285个和537个随机样本。通过比较Stimmunology分析之前和之后在样品上获得的结果与基于标准ELISA的分析方法，来对移民中的HIV患病率，发病率和窗口期长度进行分析。这种促进体外刺激抗体合成的新方法导致在窗口期诊断出1992和1998年移民群体分别被诊断为HIV感染个体的2.7％和0.36％。使用数学模型，被调查人口的窗口期长度估计为2到5个月。对早期血清阴性的HIV感染者进行的免疫学检测提供了一种可靠且一致的鉴定血清阴性的HIV-1感染者中感染的方法。将数学模型应用于获得的数据使我们能够定义窗口周期长度，发现该长度超出了先前的估计。这些结果表明需要重新评估用于评估HIV-1感染的真实发生率和流行率的技术，特别是在撒哈拉以南非洲地区的人群中。

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