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首页> 外文期刊>AIDS Research and Human Retroviruses >Short communication: Biological and genetic characterization of HIV type 1 subtype B and nonsubtype B transmitted viruses: usefulness for vaccine candidate assessment.
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Short communication: Biological and genetic characterization of HIV type 1 subtype B and nonsubtype B transmitted viruses: usefulness for vaccine candidate assessment.

机译：简短交流：HIV 1型B型亚型和非B型亚型传播病毒的生物学和遗传特征：对候选疫苗的评估有用。

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Due to the extraordinary degree of genetic diversity of HIV-1 and the structural complexity of its envelope glycoproteins, designing an effective vaccine is difficult, requiring the development of viral reagents to assess vaccine-elicited neutralizing antibodies. The aim of this study was to improve on our previously developed panel of HIV-1 strains of different genetic forms, focusing on strains from acute and recently acquired infections as the most representative of the transmitted viruses. HIV-1 primary isolates were expanded in peripheral blood mononuclear cells. Viral stocks of 40 ml each were produced. Syncytium-inducing (SI) phenotype, coreceptor use, and TCID(50)/ml were determined. Near full-length HIV-1 genomes were amplified by RT-nested PCR in four overlapping segments. Phylogenetic analyses were performed with neighbor-joining trees and bootscanning. Forty-four HIV-1 strains were included in the panel. Twenty-four (54.1%) strains were from early infections (16 acute and 8 recent); of them, 21 (87%) were sexually transmitted. NSI/R5 phenotype was detected in 37 (84.1%) viruses and SI/R5,X4 in another 7 (15.9%). TCID(50)/ml ranged between 10(4) and 10(6.6). Twelve different genetic forms constituted this panel: subtypes A1, B, C, F1, and G; circulating recombinant forms CRF02_AG, CRF14_BG, and CRF24_BG; and unique recombinant forms CRF02_AG/A3, BF1, CRF12_BF/B, and DF1G. In conclusion, in this study, we report the development of a comprehensive and well-characterized panel of HIV-1 isolates for assessing neutralization in HIV vaccine research. This panel is available for distribution through the Programme EVA Centre for AIDS Reagents, National Institute for Biological Standard and Control (NIBSC).
机译：由于HIV-1的遗传多样性非常高，并且其包膜糖蛋白的结构复杂，因此设计有效的疫苗非常困难，需要开发病毒试剂来评估疫苗引起的中和抗体。这项研究的目的是改进我们先前开发的不同遗传形式的HIV-1菌株，重点研究来自急性和最近获得的感染的菌株，作为传播病毒的最典型代表。 HIV-1主要分离株在外周血单个核细胞中扩增。产生每种40ml的病毒原液。确定了合胞体诱导（SI）表型，共受体使用和TCID（50）/ ml。通过RT巢式PCR在四个重叠片段中扩增近乎全长的HIV-1基因组。系统发育分析进行了相邻的树木和bootscanning。小组中包括44个HIV-1菌株。二十四（54.1％）个菌株来自早期感染（16例急性感染和8例近期感染）。其中21个（87％）是通过性传播的。在37种病毒（84.1％）中检测到NSI / R5表型，在另外7种病毒（15.9％）中检测到SI / R5，X4。 TCID（50）/ ml介于10（4）和10（6.6）之间。十二种不同的遗传形式构成了该小组：亚型A1，B，C，F1和G;循环重组形式CRF02_AG，CRF14_BG和CRF24_BG;和独特的重组形式CRF02_AG / A3，BF1，CRF12_BF / B和DF1G。总而言之，在这项研究中，我们报告了一个开发完整且功能齐全的HIV-1分离物小组的进展，以评估HIV疫苗研究中的中和作用。该面板可通过美国国家生物标准与控制研究所（NIBSC）的EVA计划试剂中心进行分配。

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